EIF4A3 anticorps (AA 351-411)
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- Antigène Voir toutes EIF4A3 Anticorps
- EIF4A3 (Eukaryotic Translation Initiation Factor 4A3 (EIF4A3))
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Épitope
- AA 351-411
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Reactivité
- Humain, Souris, Rat
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp EIF4A3 est non-conjugé
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Application
- Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Réactivité croisée
- Humain, Souris, Rat
- Homologie
- Dog,Cow,Horse,Chicken
- Purification
- Purified by Protein A.
- Immunogène
- KLH conjugated synthetic peptide derived from human eIF4A3
- Isotype
- IgG
- Top Product
- Discover our top product EIF4A3 Anticorps primaire
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- Indications d'application
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WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500 - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 μg/μL
- Buffer
- 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
- Agent conservateur
- ProClin
- Précaution d'utilisation
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Date de péremption
- 12 months
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- Antigène
- EIF4A3 (Eukaryotic Translation Initiation Factor 4A3 (EIF4A3))
- Autre désignation
- eIF4A3 (EIF4A3 Produits)
- Synonymes
- anticorps DDX48, anticorps NMP265, anticorps NUK34, anticorps eIF4AIII, anticorps 2400003O03Rik, anticorps Ddx48, anticorps eIF4A-III, anticorps mKIAA0111, anticorps ddx48, anticorps nuk34, anticorps nmp265, anticorps eif4aiii, anticorps EIF4A3, anticorps eif4a3, anticorps zgc:56139, anticorps XeIF-4AIII, anticorps eif4a3-B, anticorps eukaryotic translation initiation factor 4A3, anticorps eukaryotic initiation factor 4A-III, anticorps eukaryotic translation initiation factor 4A3 S homeolog, anticorps Eukaryotic initiation factor 4A-III, anticorps EIF4A3, anticorps Eif4a3, anticorps eif4a3, anticorps LOC100185906, anticorps eif4a3.S, anticorps if4a3
- Sujet
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Synonyms: Eukaryotic initiation factor 4A-III, EIF4A3, eIF-4A-III, ATP-dependent RNA helicase DDX48, ATP-dependent RNA helicase eIF4A-3, DEAD box protein 48, Eukaryotic initiation factor 4A-like NUK-34, Eukaryotic translation initiation factor 4A isoform 3, Nuclear matrix protein 265, NMP 265, hNMP 265, Eukaryotic initiation factor 4A-III, N-terminally processed, EIF4A3, DDX48, KIAA0111
Background: ATP-dependent RNA helicase. Component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Core components of the EJC, that remains bound to spliced mRNAs throughout all stages of mRNA metabolism, functions to mark the position of the exon-exon junction in the mature mRNA and thereby influences downstream processes of gene expression including mRNA splicing, nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). Constitutes at least part of the platform anchoring other EJC proteins to spliced mRNAs. Its RNA-dependent ATPase and RNA-helicase activities are induced by CASC3, but abolished in presence of the MAGOH/RBM8A heterodimer, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. The inhibition of ATPase activity by the MAGOH/RBM8A heterodimer increases the RNA-binding affinity of the EJC. Involved in translational enhancement of spliced mRNAs after formation of the 80S ribosome complex. Binds spliced mRNA in sequence-independent manner, 20-24 nucleotides upstream of mRNA exon-exon junctions. Shows higher affinity for single-stranded RNA in an ATP-bound core EJC complex than after the ATP is hydrolyzed.
- ID gène
- 9775
- UniProt
- P38919
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