PUF60 anticorps (AA 1-100) (Biotin)

N° du produit ABIN5675527

Détail du produit anti-PUF60 anticorps (Biotin)

Antigène: PUF60 (Poly-U Binding Splicing Factor 60KDa (PUF60))

Épitope: AA 1-100

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PUF60 est conjugé à/à la Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Réactivité croisée: Humain, Souris, Rat

Purification: Purified by Protein A.

Immunogène: KLH conjugated synthetic peptide derived from human PUF60

Isotype: IgG

Information d'application

Indications d'application: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at -20°C for 12 months.

Date de péremption: 12 months

Détails sur PUF60

Antigène: PUF60 (Poly-U Binding Splicing Factor 60KDa (PUF60))

Autre désignation: PUF60 (PUF60 Produits)

Synonymes: anticorps FIR, anticorps RoBPI, anticorps SIAHBP1, anticorps fc21a05, anticorps wu:fc21a05, anticorps wu:fi43a08, anticorps 2410104I19Rik, anticorps 2810454F19Rik, anticorps Siahbp1, anticorps fe37c05, anticorps repressor, anticorps si:ch211-12p8.2, anticorps si:zc12p8.2, anticorps wu:fb33e11, anticorps wu:fe37c05, anticorps zgc:86806, anticorps poly(U) binding splicing factor 60, anticorps poly-U binding splicing factor a, anticorps poly-U binding splicing factor 60, anticorps poly-U binding splicing factor b, anticorps PUF60, anticorps puf60a, anticorps Puf60, anticorps puf60b

Sujet:

Synonyms: 60 kDa poly(U)-binding-splicing factor, FBP-interacting repressor, FIR, FLJ31379, FUSE-binding protein-interacting repressor, Poly U binding splicing factor 60KDa, Poly(U)-binding-splicing factor PUF60, poly-U binding splicing factor PUF60, PUF60, PUF60_HUMAN, pyrimidine tract binding splicing factor, Ro ribonucleoprotein-binding protein 1, Ro-binding protein 1, RoBP1, RoBPI, siah binding protein 1, Siah-binding protein 1, Siah-BP1, SIAHBP1

Background: DNA- and RNA-binding protein, involved in several nuclear processes such as pre-mRNA splicing, apoptosis and transcription regulation. In association with FUBP1 regulates MYC transcription at the P2 promoter through the core-TFIIH basal transcription factor. Acts as a transcriptional repressor through the core-TFIIH basal transcription factor. Represses FUBP1-induced transcriptional activation but not basal transcription. Decreases ERCC3 helicase activity. Does not repress TFIIH-mediated transcription in xeroderma pigmentosum complementation group B (XPB) cells. Is also involved in pre-mRNA splicing. Promotes splicing of an intron with weak 3'-splice site and pyrimidine tract in a cooperative manner with U2AF2. Involved in apoptosis induction when overexpressed in HeLa cells. Isoform 6 failed to repress MYC transcription and inhibited FIR-induced apoptosis in colorectal cancer. Isoform 6 may contribute to tumor progression by enabling increased MYC expression and greater resistance to apoptosis in tumors than in normal cells. Modulates alternative splicing of several mRNAs. Binds to relaxed DNA of active promoter regions. Binds to the pyrimidine tract and 3'-splice site regions of pre-mRNA, binding is enhanced in presence of U2AF2. Binds to Y5 RNA in association with TROVE2. Binds to poly(U) RNA.

ID gène: 22627

UniProt: Q9UHX1