S-100 beta, S-100 A (alpha/beta) and S-100 B (beta/beta), not reactive with S-100 A0 (alpha/alpha), Calmodulin, Parvalbumin and other Ca-binding proteins
Réactivité croisée (Details)
Cattle, human, cat, rabbit, rat, pig
Attributs du produit
The S-100 antibody has frequently been used for the detection of melanoic tumours. In brain tumours and metastases the antibody may be used for differentiation between glia derived tissue and tissues derived from other cell types. S-100 helps in differentiation of Melanoma and Schwannoma from other soft tissue tumours. It is also found in tumours of the salivary glands. S-100 is a homo- or heterodimer filament protein, composed of an alpha and a beta chain. In normal tissues it is found in glia and ependyma of the brain. In the periphery it is found in Schwann Cells, Melanocytes and in Langerhans Cells of the skin. In lymph nodes Interdigitating Cells are labelled. S-100 A0, which is exprimed e.g. by neurons, heart muscle and monocytes, will not be detected by this monoclonal antibody. S-100 ß, S-100 A (alpha/beta) and S-100 B (beta/beta), not reactive with S-100 A0 (alpha/alpha), Calmodulin, Parvalbumin and other Ca-binding proteins.
Formaldehyde-fixed tissue should be pre-treated with 0.1 % pronase to give best results. Unmasking methods involving microwave or steam cooking are not recommended!,Skin, myelinated nerves,IHC(P),60 min at RT,(liquid conc.) Dilution 1:20 - 1:50 IHC(P)
Restrictions
For Research Use only
Format
Liquid
Buffer
stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide. The volume is sufficient for at least 100 immunohistochemical tests (100 μL working solution / test)
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.