Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Immunodiffusion (ID), Immunoprecipitation (IP), Radioimmunoassay (RIA), Dot Blot (DB)
Purification
Ammonium Sulphate Precipitation and Ion Exchange Chromatography.
Immunogène
L-Lactic dehydrogenase, LDH-1(H4) isoenzyme isolated and purified from Human erythrocytes. Freund’s complete adjuvant is used in the first step of the immunization procedure.
LDH-1
Reactivité: Lapin
ELISA, WB
Hôte: Mouton
Polyclonal
unconjugated
Indications d'application
Optimal working dilution should be determined by the investigator.
Restrictions
For Research Use only
Reconstitution
Restore by adding 1.0 mL sterile distilled water.
Concentration
10.0 mg/mL
Buffer
PBS, pH 7.2 without preservatives and foreign proteins.
Agent conservateur
Without preservative
Stock
4 °C/-20 °C
Stockage commentaire
Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.
Antigène
Lactate Dehydrogenase (4H) (LDH-1)
Autre désignation
LDH1 H4 Isozyme
Sujet
Lactate dehydrogenase is an enzyme present in a wide variety of organisms, including plants and animals. It catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. Isoforms of the enzyme include LDH 1 (4H) in the heart, LDH 2 (3H1M) in the reticuloendothelial system, LDH 3 (2H2M) in the lungs, LDH 4 (1H3M) in the kidneys and LDH 5 (4M) in the liver and striated muscle. The M and H subunits are encoded by two different genes.Synonyms: L-Lactic dehydrogenase LDH-1 Isozyme H4