Cet anticorp Nucleoside Monophosphate Kinase est conjugé à/à la Biotin
Application
Western Blotting (WB), Immunofluorescence (IF), Dot Blot (DB), Enzyme Immunoassay (EIA), Immunodiffusion (ID), Immunoprecipitation (IP), Radioimmunoassay (RIA)
Attributs du produit
Molar Ratio: Bovine/IgG ~ 5.3
Purification
Ammonium Sulphate Precipitation and Ion Exchange Chromatography
Immunogène
Nucleoside Monophosphate Kinase isolated and purified from Bovine liver. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Optimal working dilution should be determined by the investigator.
Restrictions
For Research Use only
Format
Liquid
Concentration
10.0 mg/mL
Buffer
PBS, pH 7.2 without preservatives and foreign proteins
Agent conservateur
Without preservative
Stock
4 °C/-20 °C
Stockage commentaire
Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.
Nucleoside monophosphate (NMP) kinases are ubiquitous enzymes involved in the biosynthesis of nucleotides. Each enzyme catalyses the synthesis of a nucleoside diphosphate that is, in turn, converted to a nucleoside triphosphate by a non-specific nucleoside diphosphate kinase. In prokaryotes, there are five NMP kinases, one for the phosphorylation of each NMP, whereas in eukaryotic organisms, the phosphorylation of both uridine monophosphate (UMP) and cytidine monophosphate (CMP) is carried out by a bifunctional UMP/CMP kinase (Liljelund and Lacroute, 1986).Synonyms: NMPK, Nucleoside monophosphokinase