L’anticorps anti-PHKA1 Polyclonal Mouton est utilisé pour la détection de PHKA1 dans des échantillons de Lapin. Il a été validé pour WB, IF, DB, EIA, ID, IP et RIA.
IgG fraction of polyclonal sheep antiserum to phosphorylase a from rabbit muscle. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.
Purification
Ammonium Sulphate Precipitation and Ion Exchange Chromatography
Immunogène
Phosphorylase a isolated and purified from rabbit muscle. Freund’s complete adjuvant is used in the first step of the immunization procedure.