ATP1B1 anticorps (Subunit beta)
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- Antigène Voir toutes ATP1B1 Anticorps
- ATP1B1 (ATPase, Na+/K+ Transporting, beta 1 Polypeptide (ATP1B1))
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Épitope
- Subunit beta
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Reactivité
- Rat, Arabidopsis thaliana, Porc, Spinach, Poulet, Soybean, Chlamydomonas reinhardtii, Zea mays subsp. parviglumis (Balsas teosinte), Nicotiana benthamiana, Nicotiana tabacum, Oryza sativa, Bacillus cereus, Orge, Salmon
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp ATP1B1 est non-conjugé
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Application
- Western Blotting (WB), Immunofluorescence (IF), Blue-native PAGE (BN PAGE)
- Specificité
- The anti-AtpB antibody will detect the mitochondrial form of the F1 ATP synthase subcomplex, as well as the chloroplastic CF1 ATP synthase and most known bacterial F-type ATP synthases. Peptide used for antibody production is located ina beta sheet, which is partly exposed near the surface of the AtpB protein.
- Réactivité croisée (Details)
- No cross-reactivity with: archeal V-type ATP synthase
- Attributs du produit
- Expected / apparent Molecular Weight of the Antigene: 53.9kDa (Arabidopsis thaliana), 51.7 kDa (Synechocystis PCC 6803), 53.7 kDa (Spinacia oleracea)
- Purification
- serum
- Immunogène
- KLH-conjugated synthetic peptide derived from available plant, algal (chloroplastic and mitochondrial) and bacterial sequences of beta subunits of F-type ATP synthases, including Arabidopsis thaliana chloroplastic ATP synthase subunit beta UniProt: P19366, TAIR: AtCg00480 and Arabidopsis thaliana mitochondrial ATP synthase subunit beta-1, UniProt: P83483, TAIR: At5g08670 as well as Chlamydomonas reinhardtii, UniProt: P06541 and A8IQU3
- Top Product
- Discover our top product ATP1B1 Anticorps primaire
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- Indications d'application
- Recommended Dilution: 1 : 2000 - 1 : 5 000 with standard ECL (WB), 1 : 5000 (BN-PAGE).
- Commentaires
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Blue Native gel electrophoresis (BN-PAGE) has been performed on samples solubilized with digitonin (4:1) and loaded at 100 µg/well. Gel thickness was 2 mm with 4.5-16 % gradient.Antibody is recognizing mitochondrial form of AtpB Subota el. al (2011).This antibody can be used as a loading control for bacteria, Bacillus cereus.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- For reconstitution add 10 µL of sterile water
- Conseil sur la manipulation
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Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles. - Stock
- -20 °C
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Biogenesis of mitochondria in cauliflower (Brassica oleracea var. botrytis) curds subjected to temperature stress and recovery involves regulation of the complexome, respiratory chain activity, ..." dans: Biochimica et biophysica acta, Vol. 1847, Issue 4-5, pp. 399-417, (2015) (PubMed).
: "Overexpression of HEMA1 encoding glutamyl-tRNA reductase." dans: Journal of plant physiology, (2011) (PubMed).
: "The cyanobacterium Synechocystis sp. PCC 6803 is able to express an active [FeFe]-hydrogenase without additional maturation proteins." dans: Biochemical and biophysical research communications, (2011) (PubMed).
: "Differential responses of microsomal proteins and metabolites in two contrasting cadmium (Cd)-accumulating soybean cultivars under Cd stress." dans: Amino acids, (2010) (PubMed).
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Biogenesis of mitochondria in cauliflower (Brassica oleracea var. botrytis) curds subjected to temperature stress and recovery involves regulation of the complexome, respiratory chain activity, ..." dans: Biochimica et biophysica acta, Vol. 1847, Issue 4-5, pp. 399-417, (2015) (PubMed).
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- Antigène
- ATP1B1 (ATPase, Na+/K+ Transporting, beta 1 Polypeptide (ATP1B1))
- Autre désignation
- AtpB (ATP1B1 Produits)
- Synonymes
- anticorps ATP1B, anticorps Atp4b, anticorps Atpb, anticorps Atpb-1, anticorps NKbeta1, anticorps ATPBS, anticorps atp1b1, anticorps atp1b1a, anticorps cb710, anticorps ATPase Na+/K+ transporting subunit beta 1, anticorps ATPase, Na+/K+ transporting, beta 1 polypeptide, anticorps ATPase beta chain, anticorps ATP synthase CF1 beta subunit, anticorps ATPase Na+/K+ transporting subunit beta 1 S homeolog, anticorps ATPase, Na+/K+ transporting, beta 1b polypeptide, anticorps ATP1B1, anticorps Atp1b1, anticorps atpB, anticorps atp1b1.S, anticorps atp1b1b
- Sujet
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AGI Code: At5g08670, ATCG00480
ATP synthase is the universal enzyme that synthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient. - Poids moléculaire
- 53.9 kDa (Arabidopsis thaliana), 51.7 kDa (Synechocystis PCC 6803), 53.7 kDa (Spinacia oleracea)
- UniProt
- P83483, A8IQU3, P19366, P06541
- Pathways
- Thyroid Hormone Synthesis, Ribonucleoside Biosynthetic Process, SARS-CoV-2 Protein Interactome
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