Ficin anticorps (Biotin)

N° du produit ABIN568456

Détail du produit anti-Ficin anticorps (Biotin)

Antigène: Ficin

Reactivité: Fig

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Ficin est conjugé à/à la Biotin

Application: Immunofluorescence (IF), Western Blotting (WB), Enzyme Immunoassay (EIA), Immunodiffusion (ID), Immunoprecipitation (IP), Radioimmunoassay (RIA), Dot Blot (DB)

Attributs du produit: Molar Ratio: Biotin/IgG ~ 6.0

Purification: Ammonium Sulphate Precipitation and Ion Exchange Chromatography

Immunogène: Ficin isolated and purified from fig tree latex. Freund’s complete adjuvant is used in the first step of the immunization procedure

Isotype: IgG

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Reconstitution: Restore by adding 1.0 mL of sterile distilled water

Concentration: 10.0 mg/mL

Buffer: PBS, pH 7.2 without preservatives and foreign proteins

Agent conservateur: Without preservative

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.

Détails sur Ficin

Antigène: Ficin

Abstract: Ficin Produits

Sujet: Ficin is classified as a Thiol Protease. It contains a single reactive Cysteine at its active site. The amino acid homology of the active site is similar to that of Papain. Ficin will cleave proteins at the carboxyl side of Gly, Ser, Thr, Met, Lys, Arg, Tyr, Ala, Asn, and Val. The reported Km for the chromogenic substrate pGlu-Phe-Leu-p-nitroanilide is 0.43 mM. (3) Ficin is inhibited by Iodoacetamide, Iodoacetic Acid, N-Ethylmaleimide, Mercuric Chloride, DFP (diisopropyl fluorophosphate), TLCK (N-alpha-p-Tosyl-lysine Chloromethyl ketone), and TPCK (N-Tosyl-L-Phenylalanine Chloromethyl Ketone). (1,2) Enzyme Commission (EC) Number: 3.4.22.3 Molecular Weight: 23.8 kDa (1). Extinction Coefficient: E1 % = 21.0 (280 nm) (2)