Ccl12 anticorps

N° du produit ABIN5689163

Détail du produit anti-Ccl12 anticorps

Antigène: Ccl12 (Chemokine (C-C Motif) Ligand 12 (Ccl12))

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Ccl12 est non-conjugé

Application: ELISA, Western Blotting (WB), Neutralization (Neut)

Purification: Anti-mMCP-5 specific antibody was purified by affinity chromatography employing immobilized mMCP-5 matrix.

Immunogène: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mMCP-5 (murine MCP-5).

Information d'application

Indications d'application: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of mMCP-5 (100 ng/mL), a concentration of 2.0 - 3.0 μ,g/mL of this antibody is required.

ELISA:

To detect mMCP-5 by direct ELISA (using 100 μ,g/mL of this μ,L/well antibody solution) a concentration of at least 0.5 antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mMCP-5.

Sandwich:

To detect mMCP-5 by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Murine MCP-5 (XP-5226Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mMCP-5.

Western Blot:

To detect mMCP-5 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mMCP-5 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Stock: -20 °C

Stockage commentaire: MCP-5 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Détails sur Ccl12

Antigène: Ccl12 (Chemokine (C-C Motif) Ligand 12 (Ccl12))

Autre désignation: MCP-5 (Ccl12 Produits)

Synonymes: anticorps MCP-5, anticorps Scya12, anticorps chemokine (C-C motif) ligand 12, anticorps Ccl12

Sujet: MCP-5 is a member of the b-chemokine family (C-C) of cytokines. This chemokine is strongly chemotactic for human monocytes and murine peritoneal macrophages, but is not active on mouse neutrophils and shows only a weak response on mouse eosinophils at high concentrations

ID gène: 20293

UniProt: Q62401

Pathways: Cellular Response to Molecule of Bacterial Origin