Splicing Factor 1 anticorps (AA 160-266)

N° du produit ABIN5692946

Détail du produit anti-Splicing Factor 1 anticorps

Antigène: Splicing Factor 1 (SF1)

Épitope: AA 160-266

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Splicing Factor 1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Marque: Picoband™

Réactivité croisée (Details): No cross reactivity with other proteins.

Attributs du produit: Rabbit IgG polyclonal antibody for splicing factor 1 detection. Tested with WB, IHC-P, Direct ELISA in Human,Mouse,Rat.

Immunogène: E. coli-derived human splicing factor 1 recombinant protein (Position: R160-Q266).

Information d'application

Indications d'application:

Recommended Detection Systems: Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Application Details: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry(Paraffin-embedded Section), 0.5-1 μg/mL
Direct ELISA, 0.1-0.5 μg/mL

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Détails sur Splicing Factor 1

Antigène: Splicing Factor 1 (SF1)

Autre désignation: SF1 (SF1 Produits)

Synonymes: anticorps BBP, anticorps D11S636, anticorps MBBP, anticorps ZCCHC25, anticorps ZFM1, anticorps ZNF162, anticorps BB094781, anticorps CW17R, anticorps MZFM, anticorps WBP4, anticorps Zfp162, anticorps CG5836, anticorps Dmel\\CG5836, anticorps cg5836, anticorps dSF1, anticorps p70, anticorps SF1, anticorps sf1, anticorps wu:fc09f06, anticorps znf162, anticorps MWD22.25, anticorps MWD22_25, anticorps SF-1, anticorps splicing factor 1, anticorps Splicing factor 1, anticorps splicing factor-like protein, anticorps nuclear receptor subfamily 5 group A member 1, anticorps splicing factor 1 S homeolog, anticorps SF1, anticorps Sf1, anticorps sf1, anticorps AT5G51300, anticorps NR5A1, anticorps sf1.S

Sujet:

Synonyms: Splicing factor 1, Mammalian branch point-binding protein, BBP, mBBP, Transcription factor ZFM1, Zinc finger gene in MEN1 locus, Zinc finger protein 162, SF1, ZFM1, ZNF162

Tissue Specificity: Detected in lung, ovary, adrenal gland, colon, kidney, muscle, pancreas, thyroid, placenta, brain, liver and heart.

Background: Splicing factor 1 also known as zinc finger protein 162 (ZFM162) is a protein that in humans is encoded by the SF1 gene. This gene encodes a nuclear pre-mRNA splicing factor. The encoded protein specifically recognizes the intron branch point sequence at the 3' splice site, together with the large subunit of U2 auxiliary factor (U2AF), and is required for the early stages of spliceosome assembly. It also plays a role in nuclear pre-mRNA retention and transcriptional repression. The encoded protein contains an N-terminal U2AF ligand motif, a central hnRNP K homology motif and quaking 2 region which bind a key branch-site adenosine within the branch point sequence, a zinc knuckles domain, and a C-terminal proline-rich domain. Alternative splicing results in multiple transcript variants.

UniProt: Q15637

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Maintenance of Protein Location