MYLK anticorps (AA 1441-1709)

N° du produit ABIN5693029

Détail du produit anti-MYLK anticorps

Antigène: MYLK (Myosin Light Chain Kinase (MYLK))

Épitope: AA 1441-1709

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MYLK est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Marque: Picoband™

Réactivité croisée (Details): No cross reactivity with other proteins.

Attributs du produit: Rabbit IgG polyclonal antibody for MYLK detection. Tested with WB, IHC-P, Direct ELISA in Human,Mouse,Rat.

Immunogène: E. coli-derived human MYLK recombinant protein (Position: D1441-D1709).

Information d'application

Indications d'application:

Recommended Detection Systems: Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Application Details: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry(Paraffin-embedded Section), 0.5-1 μg/mL
Direct ELISA, 0.1-0.5 μg/mL

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Détails sur MYLK

Antigène: MYLK (Myosin Light Chain Kinase (MYLK))

Autre désignation: MYLK (MYLK Produits)

Synonymes: anticorps AAT7, anticorps KRP, anticorps MLCK, anticorps MLCK1, anticorps MLCK108, anticorps MLCK210, anticorps MSTP083, anticorps MYLK1, anticorps smMLCK, anticorps 9530072E15Rik, anticorps A930019C19Rik, anticorps AW489456, anticorps Mlck, anticorps SMMLCK, anticorps mlck, anticorps fj60a06, anticorps mlck1a, anticorps mylk, anticorps wu:fj60a06, anticorps zmlck1, anticorps myosin light chain kinase, anticorps myosin, light polypeptide kinase, anticorps myosin, light chain kinase a, anticorps MYLK, anticorps Mylk, anticorps mylka

Sujet:

Synonyms: Myosin light chain kinase, smooth muscle, MLCK, s  mMLCK, Kinase-related protein, KRP, Telokin, Myosin light chain kinase, smooth muscle, deglutamylated form, MYLK, MLCK, MLCK1, MYLK1

Tissue Specificity: Smooth muscle and non-muscle isozymes are expressed in a wide variety of adult and fetal tissues and in cultured endothelium with qualitative expression appearing to be neither tissue- nor development-specific. Non-muscle isoform 2 is the dominant splice variant expressed in various tissues. Telokin has been found in a wide variety of adult and fetal tissues. Accumulates in well differentiated enterocytes of the intestinal epithelium in response to tumor necrosis factor (TNF).

Background: Myosin light chain kinase, smooth muscle also known as kinase-related protein (KRP) or telokin is an enzyme that in humans is encoded by the MYLK gene. This gene, a muscle member of the immunoglobulin gene superfamily, encodes myosin light chain kinase which is a calcium/calmodulin dependent enzyme. This kinase phosphorylates myosin regulatory light chains to facilitate myosin interaction with actin filaments to produce contractile activity. This gene encodes both smooth muscle and nonmuscle isoforms. In addition, using a separate promoter in an intron in the 3' region, it encodes telokin, a small protein identical in sequence to the C-terminus of myosin light chain kinase, that is independently expressed in smooth muscle and functions to stabilize unphosphorylated myosin filaments. A pseudogene is located on the p arm of chromosome 3. Four transcript variants that produce four isoforms of the calcium/calmodulin dependent enzyme have been identified as well as two transcripts that produce two isoforms of telokin.

UniProt: Q15746