RNF43 anticorps (AA 24-197)

N° du produit ABIN5693038

Détail du produit anti-RNF43 anticorps

Antigène: RNF43 (Ring Finger Protein 43 (RNF43))

Épitope: AA 24-197

Reactivité: Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp RNF43 est non-conjugé

Application: ELISA, Western Blotting (WB)

Marque: Picoband™

Réactivité croisée (Details): No cross reactivity with other proteins.

Attributs du produit: Rabbit IgG polyclonal antibody for RNF43 detection. Tested with WB, Direct ELISA in Human,Mouse,Rat.

Immunogène: E. coli-derived human RNF43 recombinant protein (Position: G24-Y197).

Information d'application

Indications d'application:

Recommended Detection Systems: Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot.

Application Details: Western blot,0.1-0.5 μg/mL
Direct ELISA,0.1-0.5 μg/mL

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Détails sur RNF43

Antigène: RNF43 (Ring Finger Protein 43 (RNF43))

Autre désignation: RNF43 (RNF43 Produits)

Synonymes: anticorps RNF124, anticorps URCC, anticorps 4732452J19Rik, anticorps RGD1305204, anticorps ring finger protein 43, anticorps RNF43, anticorps Rnf43

Sujet:

Synonyms: E3 ubiquitin-protein ligase RNF43, RING finger protein 43, RING-type E3 ubiquitin transferase RNF43, RNF43

Tissue Specificity: Expressed in fetal kidney, fetal lung, in colon cancer tissues, hepatocellular carcinomas and lung adenocarcinomas. Overexpressed in colorectal cancer cell lines.

Background: RNF43 is a HAP95 (AKAP8L) binding ubiquitin ligase that promotes cell growth and is upregulated in colon cancer. The protein encoded by this gene is a RING-type E3 ubiquitin ligase and is predicted to contain a transmembrane domain, a protease-associated domain, an ectodomain, and a cytoplasmic RING domain. This protein is thought to negatively regulate Wnt signaling, and expression of this gene results in an increase in ubiquitination of frizzled receptors, an alteration in their subcellular distribution, resulting in reduced surface levels of these receptors. Mutations in this gene have been reported in multiple tumor cells, including colorectal and endometrial cancers. Alternative splicing results in multiple transcript variants encoding different isoforms.

Pathways: Signalisation WNT