ITM2B anticorps (Internal Region)
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- Antigène Voir toutes ITM2B Anticorps
- ITM2B (Integral Membrane Protein 2B (ITM2B))
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Épitope
- Internal Region
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Reactivité
- Humain
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Hôte
- Chèvre
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp ITM2B est non-conjugé
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Application
- ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)
- Fonction
- ITM2B
- Séquence
- HDKETYKLQR RETIK
- Réactivité croisée
- Boeuf (Vache), Humain, Souris, Rat
- Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Classe de qualité
- Verified
- Immunogène
- Peptide with sequence C-HDKETYKLQRRETIK, from the internal region of the protein sequence according to NP_068839.1.
- Isotype
- IgG
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- Indications d'application
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Immunohistochemistry: Paraffin embedded Human Small Intestine. Recommended concentration: 2.5 μg/mL.
Western Blot: Approx. 30-35 kDa band observed in HepG2 cell lysates (calculated MW of 30.3 kDa according to NP_068839.1). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation 1 hour at room temperature. Preliminary testing was unsuccessful on Rat an
Peptide ELISA: antibody detection limit dilution 1:8000.
- Commentaires
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Immunofluorescence: Strong expression of the protein seen in the cytoplasm and Golgi apparatus of A431 and HeLa cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of HeLa cells. Recomme
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- Minimize freezing and thawing.
- Stock
- -20 °C
- Stockage commentaire
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Antigène
- ITM2B (Integral Membrane Protein 2B (ITM2B))
- Autre désignation
- ITM2B (ITM2B Produits)
- Sujet
- ITM2B, integral membrane protein 2B, ABRI, BRI, BRI2, BRICD2B, E25B, E3-16, FBD, BRICHOS domain containing 2B, OTTHUMP00000018394
- ID gène
- 9445, 16432, 290364
- NCBI Accession
- NP_068839
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