IL-18 anticorps (Internal Region)

N° du produit ABIN570987

Détail du produit anti-IL-18 anticorps

Antigène: IL-18 (IL18) (Interleukin 18 (IL18))

Épitope: Internal Region

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp IL-18 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Fonction: IL18

Séquence: NPPDNIKDTK SDI

Specificité: This antibody is expected to recognise both reported isoforms (NP_001553.1, NP_001230140.1).

Réactivité croisée: Humain

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Classe de qualité: Verified

Immunogène: Peptide with sequence C-NPPDNIKDTKSDI, from the internal region of the protein sequence according to NP_001553.1, NP_001230140.1.

Isotype: IgG

Information d'application

Indications d'application:

Western Blot: Approx 23 kDa band observed in lysates of cell line HeLa and approx. 21-22 kDa band in lysates of cell lines A431 and U2OS (calculated MW of 22.3 kDa according to NP_001553.1). Recommended concentration: 1-3 μg/mL. Primary incubation 1 hour at

Peptide ELISA: antibody detection limit dilution 1:32000.

Commentaires:

Immunofluorescence: Strong expression of the protein seen in the golgi and cytoplasm of A431and U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended conce

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur IL-18

Antigène: IL-18 (IL18) (Interleukin 18 (IL18))

Autre désignation: IL18 (IL18 Produits)

Sujet: IL18, interleukin 18 (interferon-gamma-inducing factor), IGIF, IL-18, IL-1g, IL1F4, MGC12320, IL-1 gamma, interferon-gamma-inducing factor, interleukin 18, interleukin-1 gamma, interleukin-18

ID gène: 3606, 29197

NCBI Accession: NP_001553, NP_001230140

Pathways: Cellular Response to Molecule of Bacterial Origin, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome