IL-18 anticorps (Internal Region)
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- Antigène Voir toutes IL-18 (IL18) Anticorps
- IL-18 (IL18) (Interleukin 18 (IL18))
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Épitope
- Internal Region
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Reactivité
- Humain
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Hôte
- Chèvre
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp IL-18 est non-conjugé
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Application
- Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)
- Fonction
- IL18
- Séquence
- NPPDNIKDTK SDI
- Specificité
- This antibody is expected to recognise both reported isoforms (NP_001553.1, NP_001230140.1).
- Réactivité croisée
- Humain
- Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Classe de qualité
- Verified
- Immunogène
- Peptide with sequence C-NPPDNIKDTKSDI, from the internal region of the protein sequence according to NP_001553.1, NP_001230140.1.
- Isotype
- IgG
- Top Product
- Discover our top product IL18 Anticorps primaire
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- Indications d'application
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Western Blot: Approx 23 kDa band observed in lysates of cell line HeLa and approx. 21-22 kDa band in lysates of cell lines A431 and U2OS (calculated MW of 22.3 kDa according to NP_001553.1). Recommended concentration: 1-3 μg/mL. Primary incubation 1 hour at
Peptide ELISA: antibody detection limit dilution 1:32000.
- Commentaires
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Immunofluorescence: Strong expression of the protein seen in the golgi and cytoplasm of A431and U2OS cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended conce
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- Minimize freezing and thawing.
- Stock
- -20 °C
- Stockage commentaire
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Antigène
- IL-18 (IL18) (Interleukin 18 (IL18))
- Autre désignation
- IL18 (IL18 Produits)
- Sujet
- IL18, interleukin 18 (interferon-gamma-inducing factor), IGIF, IL-18, IL-1g, IL1F4, MGC12320, IL-1 gamma, interferon-gamma-inducing factor, interleukin 18, interleukin-1 gamma, interleukin-18
- ID gène
- 3606, 29197
- NCBI Accession
- NP_001553, NP_001230140
- Pathways
- Cellular Response to Molecule of Bacterial Origin, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome
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