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Glutathione anticorps

WB, IP, IA Hôte: Souris Monoclonal L4H unconjugated
N° du produit ABIN577695
  • Antigène Voir toutes Glutathione Anticorps
    Glutathione
    Reactivité
    Veuillez nous consulter SVP
    Hôte
    • 10
    • 5
    Souris
    Clonalité
    • 8
    • 7
    Monoclonal
    Conjugué
    • 8
    • 3
    • 2
    • 1
    • 1
    Cet anticorp Glutathione est non-conjugé
    Application
    • 12
    • 6
    • 6
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunoprecipitation (IP), Immunoassay (IA)
    Marque
    AbX™
    Attributs du produit
    Designed for Immunofluorescence and FACS Analysis
    Supplied as 50 µg in PBS
    For use under Non-Reducing conditions
    Purification
    Protein A Purified
    Immunogène
    Glutathione conjugated to Keyhole Limpet Hemocyanin
    Clone
    L4H
    Isotype
    IgG2a
    Top Product
    Discover our top product Glutathione Anticorps primaire
  • Indications d'application
    Western blotting, Immunoassay, and Immunoprecipitation
    Western blotting, 1:1,000 151119 AbX™ Anti-Glutathione Mouse Monoclonal
    Detection of protein-glutathione adducts on Western Blot under non-reducing conditions. PTP-1B refers to phosphotyrosine phosphatase 1B
    HeLa cells incubated with Glutathione monoclonal, clone L4H, at a 1:100 dilution, counterstained with FITC- labeled goat anti-mouse IgG. Red nuclear stain is DAPI.
    Commentaires

    Applications include Western blotting, Immunoassay and Immunoprecipitation Supplied in PBS with no carrier protein For use under Non-Reducing conditions

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    100 μg/mL
    Buffer
    Phosphate Buffered Saline at pH 7.2 containing 0.09 % Sodium Azide
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
    Stock
    4 °C
    Stockage commentaire
    Short Term: 4°C. Extended: Aliquot and freeze at -20°C
  • Antigène
    Glutathione
    Abstract
    Glutathione Produits
    Synonymes
    anticorps GT, anticorps ILBP, anticorps ILLBP, anticorps PIP, anticorps I-15P, anticorps I-BABP, anticorps ILBP3, anticorps Illbp, anticorps I-BALB, anticorps I-BAP, anticorps fatty acid binding protein 6, anticorps fatty acid binding protein 6, ileal (gastrotropin), anticorps FABP6, anticorps Fabp6
    Classe de substances
    Chemical
    Sujet
    Glutathione (GSH) is the highest concentration non-protein thiol in mammalian cells and is present in concentrations of 0.5 to 10 mM. GSH plays a key role in many biological processes, including the synthesis of proteins and DNA, the transport of amino acids, and the protection of cells against oxidation. Harmful hydrogen peroxide cellular levels are minimized by the enzyme glutathione peroxidase (GP) using GSH as a reductant. The oxidized GSH dimer, GSSG, is formed from GSH and peroxide by the GP reaction. An important role of GSSG in the NFkB activating signal cascade is suggested by the fact that the potent NFkB inducer TPA increases intracellular GSSG levels and GSSG/GSH ratios. The AbX™ Glutathione Mouse Monoclonal Antibody is produced as a Protein A purified antibody. It will measure GSH-protein complexes under non-reducing conditions.
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