Vinculin anticorps

N° du produit ABIN604849

Détail du produit anti-Vinculin anticorps

Antigène: Vinculin (VCL)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Vinculin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marque: IHC-plus™

Specificité: Recognizes human vinculin.

Purification: Protein G purified

Immunogène: Semi-purified vinculin. MW of Antigen: 130kD (vinculin) and 150kD (meta-vinculin). Cellular Localization: Cell membrane and cytoplasmic.

Type of Immunogen: Protein extract

Isotype: IgG1

Information d'application

Indications d'application: Approved: IF, IHC, IHC-P (2.5 - 5 μg/mL), IP, WB (1 - 2 μg/mL)

Usage: Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation, Immunohistochemistry and Studies of vinculin in platelet activation. Immunoprecipitation (Denatured): 2 μg/mg protein lysate (Use Protein G). Western Blot: 1-2 μg/mL for 2hrs at RT. Staining of formalin/paraffin tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4. No preservatives or stabilizing proteins added.

Agent conservateur: Azide free

Conseil sur la manipulation: avoid freeze thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Détails sur Vinculin

Antigène: Vinculin (VCL)

Autre désignation: VCL / Vinculin (VCL Produits)

Synonymes: anticorps CMD1W, anticorps CMH15, anticorps MV, anticorps MVCL, anticorps VCL, anticorps im:6902790, anticorps hm:zehn2160, anticorps DDBDRAFT_0186762, anticorps DDBDRAFT_0232320, anticorps DDB_0186762, anticorps DDB_0232320, anticorps 9430097D22, anticorps AA571387, anticorps AI462105, anticorps AW545629, anticorps CG3299, anticorps CT11081, anticorps Dmel\\CG3299, anticorps Dmvincp, anticorps EG:103B4.1, anticorps Vin, anticorps Vin2EF, anticorps vinc, anticorps LOC100228062, anticorps vcl-A, anticorps vinculin, anticorps vinculin a, anticorps vinculin A, anticorps Vinculin, anticorps vinculin S homeolog, anticorps VCL, anticorps vcla, anticorps vinA, anticorps Vcl, anticorps Vinc, anticorps CpipJ_CPIJ013873, anticorps Bm1_37935, anticorps vcl, anticorps vcl.S

Sujet: Name/Gene ID: VCL
Family: Cytoskeleton

Synonyms: VCL, CMD1W, CMH15, Metavinculin, Vinculin, MVCL

ID gène: 7414

UniProt: P18206

Pathways: Cell-Cell Junction Organization, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2