CD44 Standard anticorps (CF®594)
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- Antigène Voir toutes CD44 Standard (CD44s) Anticorps
- CD44 Standard (CD44s)
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Reactivité
- Humain, Non-Human Primate
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp CD44 Standard est conjugé à/à la CF®594
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Application
- Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Fonction
- Mouse Monoclonal anti-CD44 Standard (HCAM/918), CF594 Conjugate
- Attributs du produit
- This antibody recognizes a cell surface glycoprotein of 80-95 kDa (CD44) on lymphocytes, monocytes, and granulocytes (Leucocyte Typing Workshop V). Its epitope is resistant to digestion by trypsin and chymotrypsin. The CD44 family of glycoproteins exists in a number of variant isoforms, the most common being the standard 85-95 kDa or hematopoietic variant (CD44s). Higher molecular weight isoforms are described in epithelial cells (CD44v), which are believed to function in intercellular adhesion and stromal binding. CD44 immunostaining is commonly used for the discrimination of urothelial transitional cell carcinoma in-situ from non-neoplastic changes in the urothelium. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Immunogène
- Recombinant human HCAM protein
- Clone
- HCAM-918
- Isotype
- IgG2a kappa
- Top Product
- Discover our top product CD44s Anticorps primaire
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- Indications d'application
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Immunohistology formalin-fixed 0.25-0.5 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
- Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Optimal dilution for a specific application should be determined by user
- Commentaires
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DU145 cells. Tonsil, lymph node or breast carcinomas.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 100 μg/mL
- Buffer
- PBS/0.1 % BSA/0.05 % azide
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- Protect from light
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- Antigène
- CD44 Standard (CD44s)
- Autre désignation
- CD44 Standard (CD44s Produits)
- Poids moléculaire
- 80-95 kDa
- ID gène
- 960, 502328
- UniProt
- P16070
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