Blood Group Lewis B anticorps

N° du produit ABIN6153110

Détail du produit anti-Blood Group Lewis B anticorps

Antigène: Blood Group Lewis B

Reactivité: Humain, Cobaye, Porc

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Blood Group Lewis B est non-conjugé

Application: Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunostaining (ISt)

Fonction: Mouse Monoclonal anti-Lewis-B (2-25LE), Purified, BSA-free

Attributs du produit: The Lewis histo-blood group system comprises a set of fucosylated glycosphingolipids that are synthesized by exocrine epithelial cells and circulate in body fluids. The glycosphingolipids function in embryogenesis, tissue differentiation, tumor metastasis, inflammation, and bacterial adhesion. They are secondarily absorbed to red blood cells giving rise to their Lewis phenotype. This gene is a member of the fucosyltransferase family, which catalyzes the addition of fucose to precursor polysaccharides in the last step of Lewis antigen biosynthesis. It encodes an enzyme with alpha(1,3)-fucosyltransferase and alpha(1,4)-fucosyltransferase activities. Lewis blood group antigens are carbohydrate moieties structurally integrated in mucous secretions. Lewis antigen system alterations have been described in gastric carcinoma and associated lesions. Anomalous expression of Lewis B antigen has been found in some non-secretory gastric carcinomas and colorectal cancers. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Purification: Purified

Immunogène: Mucin isolated from a human ovarian cyst fluid

Clone: 2-25LE

Isotype: IgG1 kappa

Information d'application

Indications d'application: Immunohistology formalin-fixed 0.5-1 μg/mL

• Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Commentaires:

Colon

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS (no BSA, no azide)

Agent conservateur: Azide free

Détails sur Blood Group Lewis B

Antigène: Blood Group Lewis B

Autre désignation: Lewis-B (Blood Group Lewis B Produits)

Synonymes: anticorps MUC5, anticorps TBM, anticorps leB, anticorps mucin 5AC, oligomeric mucus/gel-forming, anticorps MUC5AC

Poids moléculaire: 45 kDa

ID gène: 2525, 169238

UniProt: P21217