Melanoma Marker anticorps (CF®488A)

N° du produit ABIN6157161

Détail du produit anti-Melanoma Marker anticorps (CF®488A)

Antigène: Melanoma Marker

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Melanoma Marker est conjugé à/à la CF®488A

Application: Immunohistochemistry (IHC), Immunofluorescence (IF)

Fonction: Mouse Monoclonal anti-Melanoma Marker (KBA.62), CF488A Conjugate

Attributs du produit: KBA.62 is a novel anti-melanoma antibody. It reacts positively against melanocytic tumors but not other tumors, thus demonstrating specificity and sensitivity. Moreover, it reacts positively against junctional nevus cells but not intradermal nevi, and against fetal melanocytes but not normal adult melanocytes. KBA.62 antibody is useful in identifying malignant melanomas. Metastatic amelanotic melanoma can often be confused with a variety of poorly differentiated carcinomas, large cell lymphomas, sarcomas, spindle cell carcinomas and various types of mesenchymal neoplasms. A keratin-negative, vimentin-rich neoplasm that immuno-reacts with antibody to S-100 protein and with KBA.62 antibody is, with rare exception, a melanoma. Anti-KBA.62 is a useful additional marker for melanoma, specifically in desmoplastic/spindle cell cases and in the context of micro-metastasis in sentinel lymph node. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Human KAL cells derived from lymph node metastasis of malignant melanoma

Clone: KBA-62

Isotype: IgG1 kappa

Information d'application

Indications d'application: Immunohistology (formalin)

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Commentaires:

SK-MEL-13 and SK-MEL-19 Melanoma cell lines, Melanomas

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Détails sur Melanoma Marker

Antigène: Melanoma Marker

Abstract: Melanoma Marker Produits

Sujet: Human Melanoma Associated Antigen, KBA.62

Poids moléculaire: Multiple (140, 135 and 128 kDa and two weak bands of 88 and 73 kDa)