KRIT1 anticorps (N-Term)

N° du produit ABIN616004

Détail du produit anti-KRIT1 anticorps

Antigène: KRIT1 (KRIT1, Ankyrin Repeat Containing (KRIT1))

Épitope: AA 1-736, N-Term

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp KRIT1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Specificité: This antibody is anti-His depleated. It detects KRIT1 / CCM1.

Réactivité croisée (Details): Species reactivity (tested):Human.

Purification: Protein A Chromatography

Immunogène: Highly pure (>95%) recombinant Human CCM-1 (Met1-Ser736) derived from E. coli fused to a C-teminal His-tag (6 x His) (Cat.-No AR26002PU-N)

Isotype: IgG

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL. Centrifuge vial prior to opening.

Buffer: 5 mM PBS, pH 7.2

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur KRIT1

Antigène: KRIT1 (KRIT1, Ankyrin Repeat Containing (KRIT1))

Autre désignation: KRIT1 / CCM1 (KRIT1 Produits)

Synonymes: anticorps CAM, anticorps CCM1, anticorps 2010007K12Rik, anticorps A630036P20Rik, anticorps AA432855, anticorps AI450393, anticorps AI643869, anticorps BB155247, anticorps BB235701, anticorps Ccm1, anticorps RGD1305929, anticorps krit1, anticorps fb36f07, anticorps san, anticorps santa, anticorps wu:fb36f07, anticorps zgc:63585, anticorps KRIT1, ankyrin repeat containing, anticorps KRIT1, anticorps Krit1, anticorps krit1

Sujet: Cerebral Cavernous Malformations (CCM) are frequent vascular abnormalities caused by mutations in one of the CCM genes. CCM-1 (also known as KRIT1) stabilizes endothelial junctions and is essential for vascular morphogenesis in mouse embryos. However, cellular functions of CCM-1 during the early steps of the CCM pathogenesis remain unknown. It was shown that CCM-1 represents an antiangiogenic protein to keep the human endothelium quiescent. CCM-1 inhibits endothelial proliferation, apoptosis, migration, lumen formation, and sprouting angiogenesis in primary human endothelial cells. CCM-1 strongly induces DLL4-NOTCH signaling, which promotes AKT phosphorylation but reduces phosphorylation of the mitogen-activated protein kinase ERK. Consistently, blocking of NOTCH activity alleviates CCM-1 effects. ERK phosphorylation is increased in human CCM lesions. Transplantation of CCM-1-silenced human endothelial cells into SCID mice recapitulates hallmarks of the CCM pathology and serves as a unique CCM model system.Synonyms: Krev interaction trapped 1

ID gène: 889

NCBI Accession: NP_004903

UniProt: O00522

Pathways: Cell RedoxHomeostasis