CD99 anticorps (CF®647)
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- Antigène Voir toutes CD99 Anticorps
- CD99
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Reactivité
- Humain
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp CD99 est conjugé à/à la CF®647
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Application
- Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Fonction
- Mouse Monoclonal anti-CD99 (MIC2/877), CF647 Conjugate
- Attributs du produit
- This antibody recognizes a sialoglycoprotein of 27-32 kDa, identified as CD99, or MIC2 gene product, or E2 antigen. MIC2 gene is located in the pseudo-autosomal region of the human X and Y chromosome. MIC2 gene encodes two distinct proteins, which are produced by alternative splicing of the CD99 gene transcript and are identified as bands of 30 and 32 kDa (p30/32).Although its function is not fully understood, CD99 is implicated in various cellular processes including homotypic aggregation of T cells, upregulation of T cell receptor and MHS molecules, apoptosis of immature thymocytes and leukocyte diapedesis.CD99 is expressed on the cell membrane of some lymphocytes, cortical thymocytes, and granulosa cells of the ovary. Most pancreatic islet cells, Sertoli cells of the testis, and some endothelial cells express this antigen. Mature granulocytes express very little or no CD99. MIC2 is strongly expressed on Ewing s sarcoma cells and primitive peripheral neuroectodermal tumors. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Immunogène
- Recombinant full-length human MIC2 protein
- Clone
- MIC2-877
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product CD99 Anticorps primaire
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- Indications d'application
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Immunohistology formalin-fixed 1-2 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
- Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Does not react with rat, others not tested
- Optimal dilution for a specific application should be determined by user
- Commentaires
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MOLT-4 cells. Pancreas or Ewing s sarcoma
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 100 μg/mL
- Buffer
- PBS/0.1 % BSA/0.05 % azide
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- Protect from light
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- Antigène
- CD99
- Autre désignation
- CD99 (CD99 Produits)
- Synonymes
- anticorps 1110061M03Rik, anticorps 2410026K10Rik, anticorps D4, anticorps Pilr-l, anticorps pilr-1, anticorps HBA71, anticorps MIC2, anticorps MIC2X, anticorps MIC2Y, anticorps MSK5X, anticorps CD99 antigen, anticorps CD99 molecule (Xg blood group), anticorps CD99 molecule, anticorps Cd99, anticorps CD99
- Classe de substances
- Viral Protein
- Sujet
- 12E7, E2 antigen, MIC 2X, MIC 2Y, MIC2, Protein MIC2, Surface antigen MIC2, T-cell surface glycoprotein E2
- Poids moléculaire
- 27-32 kDa
- ID gène
- 4267, 653349
- UniProt
- P14209
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