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Actin, Muscle Specific anticorps (CF®568)

Reactivité: Humain, Lapin, Rat FACS, IF, IHC (f), WB Hôte: Souris Monoclonal MSA-953 CF®568
N° du produit ABIN6162587
  • Antigène Tous les produits Actin, Muscle Specific
    Actin, Muscle Specific
    Reactivité
    • 27
    • 26
    • 26
    • 9
    • 9
    • 9
    • 9
    Humain, Lapin, Rat
    Hôte
    • 27
    Souris
    Clonalité
    • 27
    Monoclonal
    Conjugué
    • 7
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    Cet anticorp Actin, Muscle Specific est conjugé à/à la CF®568
    Application
    • 26
    • 26
    • 17
    • 8
    • 2
    • 2
    • 1
    Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Western Blotting (WB)
    Fonction
    Mouse Monoclonal anti-Muscle-Specific-Actin (MSA/953), CF568 Conjugate
    Attributs du produit
    This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Anti-muscle specific actin recognizes alpha and gamma isotypes of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative.It stains tumors of smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas). Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
    Immunogène
    Synthetic peptide common among human muscle actins
    Clone
    MSA-953
    Isotype
    IgG1 kappa
  • Indications d'application
    Immunohistology formalin-fixed 0.25-0.5 μg/mL
    • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
    • Immunofluorescence 0.5-1 μg/mL
    • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
    • Western blotting 0.5-1.0 μg/mL
    • Optimal dilution for a specific application should be determined by user
    Commentaires

    Muscle or sarcoma

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    100 μg/mL
    Buffer
    PBS/0.1 % BSA/0.05 % azide
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    Protect from light
  • Antigène
    Actin, Muscle Specific
    Autre désignation
    Muscle-Specific-Actin (Actin, Muscle Specific Produits)
    Sujet
    ACTA, ACTA1, ACTA2, ACTC1, Actin, ACTSA, Alpha-2 actin, alpha skeletal muscle, Alpha-actin-1, Cardiac muscle alpha actin-1, Skeletal muscle alpha actin-1
    Poids moléculaire
    Multiple
    ID gène
    58, 59, 70
    UniProt
    P62736
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