ICAM-3/CD50 anticorps
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- Antigène Voir toutes ICAM-3/CD50 (ICAM3) Anticorps
- ICAM-3/CD50 (ICAM3) (Intercellular Adhesion Molecule 3 (ICAM3))
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Reactivité
- Humain
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp ICAM-3/CD50 est non-conjugé
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Application
- Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Fonction
- Mouse Monoclonal anti-CD50 (ICAM-3) (101-1D2), Purified, with BSA
- Attributs du produit
- This antibody recognizes an N-glycosylated glycoprotein of 120 kDa with intra-chain disulfide bonds, identified as CD50 or ICAM-3 (WS: IV & V). Its epitope localizes in the D2 extracellular domain and is resistant to neuraminidase and proteases. CD50 is the major ligand for LFA-1 (CD11a/CD18) and may have signalling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C. This MAb inhibits primary mixed lymphocyte culture (MLC) but not secondary MLC, cytotoxicity or proliferation induced by mitogens. It blocks binding of NK1-L16 stimulated T cells to L cells expressing CD50. This MAb is excellent for staining of formalin/paraffin tissues. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Purification
- Purified
- Immunogène
- Stimulated human leukocytes
- Clone
- 101-1D2
- Isotype
- IgG2a kappa
- Top Product
- Discover our top product ICAM3 Anticorps primaire
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- Indications d'application
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Immunohistology formalin-fixed 0.5-1 μg/mL
- Staining of formalin/paraffin tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
- Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Optimal dilution for a specific application should be determined by user
- Commentaires
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HL-60 or THP-1 cells. Lymph node and tonsil
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 200 μg/mL
- Buffer
- PBS/0.05 % BSA/0.05 % azide
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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- Antigène
- ICAM-3/CD50 (ICAM3) (Intercellular Adhesion Molecule 3 (ICAM3))
- Autre désignation
- CD50 (ICAM-3) (ICAM3 Produits)
- Synonymes
- anticorps CD50, anticorps CDW50, anticorps ICAM-R, anticorps ICAM-3, anticorps ICAM3, anticorps intercellular adhesion molecule 3, anticorps ICAM3, anticorps Icam3
- Sujet
- ICAMR, Intercellular adhesion molecule 3 (ICAM3)
- Poids moléculaire
- 110-160 kDa
- ID gène
- 3385, 354563
- UniProt
- P32942
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