Keratin 10 anticorps (CF®640R)
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- Antigène Voir toutes Keratin 10 (KRT10) Anticorps
- Keratin 10 (KRT10)
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Reactivité
- Humain, Chien, Cat
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp Keratin 10 est conjugé à/à la CF®640R
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Application
- Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)
- Fonction
- Mouse Monoclonal anti-Cytokeratin 10 (DE-K10), CF640R Conjugate
- Attributs du produit
- This MAb recognizes a protein of 56.5 kDa, identified as cytokeratin 10 (CK10). CK10 is expressed in all suprabasal layers of the epidermis. In the epidermis, expression of CK10 strictly parallels the extent of differentiation, it is absent in the basal layer, appears in the first suprabasal layers and increases in concentration towards the granular layer. However, CK10 is rarely detected in early stages of vulvar squamous carcinomas (tumors less than 2 cm, clinical stage I) regardless of the tumor grade. In larger and more advanced tumors (greater than 2 cm, clinical stages II and III), CK10 is detected very frequently. Expression of CK10 is related to maturation of malignant keratinocytes, being preferentially detected in more-differentiated parts. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Immunogène
- Cytoskeletal preparation extracted from human ectocervical epithelium
- Clone
- DE-K10
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product KRT10 Anticorps primaire
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- Indications d'application
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Flow cytometry 0.5-1 μg/million cells in 0.1 mL
- Immunohistology (formalin) 0.1-0.2 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
- Immunofluorescence 0.5-1 μg/mL
- Optimal dilution for a specific application should be determined by user
- Commentaires
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A431, HeLa, MCF7 cells. Esophagus or tonsil.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 100 μg/mL
- Buffer
- PBS/0.1 % BSA/0.05 % azide
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- Protect from light
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- Antigène
- Keratin 10 (KRT10)
- Autre désignation
- Cytokeratin 10 (KRT10 Produits)
- Synonymes
- anticorps BCIE, anticorps BIE, anticorps CK10, anticorps EHK, anticorps K10, anticorps KPP, anticorps D130054E02Rik, anticorps K1C1, anticorps Krt-1.10, anticorps Krt1-10, anticorps Ka10, anticorps Ker10, anticorps KRT10, anticorps KRT25, anticorps KRT27, anticorps KRT28, anticorps keratin 10, anticorps keratin 10, type I S homeolog, anticorps keratin, type I cytoskeletal 25, anticorps KRT10, anticorps Krt10, anticorps krt10.S, anticorps LOC101085587
- Sujet
- BCIE, BIE, EHK, Keratin Type I Cytoskeletal 10, KRT10
- Poids moléculaire
- 56.5 kDa
- ID gène
- 3858, 99936
- UniProt
- P13645
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