HLA-DRB1 anticorps
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- Antigène Voir toutes HLA-DRB1 Anticorps
- HLA-DRB1 (Major Histocompatibility Complex, Class II, DR beta 1 (HLA-DRB1))
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Reactivité
- Humain, Singe
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp HLA-DRB1 est non-conjugé
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Application
- Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunostaining (ISt), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Fonction
- Mouse Monoclonal anti-HLA-DRB (HLA-DRB/1067), Purified, BSA-free
- Attributs du produit
- This MAb reacts with the beta-chain of HLA-DRB1 antigen, a member of MHC class II molecules. It does not cross react with HLA-DP and HLA-DQ. Its epitope is different from that of MAb L243. HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36 kDa alpha (heavy) chain and a 28 kDa beta (light) chain. It is expressed on B-cells, activated T-cells, monocytes/macrophages, dendritic cells and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 Molecules, HLA-DR is critical for efficient peptide presentation to CD4 T cells. It is an excellent histiocytic marker in paraffin sections producing intense cytoplasmic staining. True histiocytic neoplasms are similarly positive. HLA-DR antigens also occur on a variety of epithelial cells and their corresponding neoplastic counterparts. Loss of HLA-DR expression is related to tumor microenvironment and predicts adverse outcome in diffuse large B-cell lymphoma. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Purification
- Purified
- Immunogène
- Activated human peripheral blood mononuclear cells
- Clone
- HLA-DRB-1067
- Isotype
- IgG2b kappa
- Top Product
- Discover our top product HLA-DRB1 Anticorps primaire
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- Indications d'application
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Immunohistology formalin-fixed 0.25-0.5 μg/mL
- Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
- Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Western blotting 0.5-1 μg/mL
- Optimal dilution for a specific application should be determined by user
- Commentaires
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Ramos, Daudi or HuT78 cells. Tonsil or lymph node
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- PBS (no BSA, no azide)
- Agent conservateur
- Azide free
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- Antigène
- HLA-DRB1 (Major Histocompatibility Complex, Class II, DR beta 1 (HLA-DRB1))
- Autre désignation
- HLA-DRB (HLA-DRB1 Produits)
- Synonymes
- anticorps MGC108235, anticorps DRB1, anticorps DRw10, anticorps HLA-DR1B, anticorps HLA-DRB, anticorps SS1, anticorps major histocompatibility complex, class II, DR beta 1, anticorps hla-drb1, anticorps HLA-DRB1
- Poids moléculaire
- 28 kDa (beta chain)
- ID gène
- 3123, 534322
- UniProt
- P01911
- Pathways
- TCR Signaling, Positive Regulation of Peptide Hormone Secretion, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events, Cancer Immune Checkpoints, Human Leukocyte Antigen (HLA) in Adaptive Immune Response
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