Ku70 + Ku80 anticorps

N° du produit ABIN6251789

Détail du produit anti-Ku70 + Ku80 anticorps

Antigène: Ku70 + Ku80 (KU80) (CG18801 Gene Product From Transcript CG18801-RA (KU80))

Reactivité: Humain, Primate

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Ku70 + Ku80 est non-conjugé

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificité: Does not react with mouse, rat, chicken

Purification: Protein G purified monoclonal antibody

Immunogène: Nuclear extract of human HL-60 cells was used as the immunogen for this Ku70 + Ku80 antibody.

Clone: KU729

Isotype: IgG1 kappa

Information d'application

Indications d'application: Flow cytometry: 0.5-1 μg/10e6 cells

IF: 0.5-1.0 μg/mL

Immunocytochemistry (Acetone-fixed cells): 0.25-0.5 μg/mL for 30 minutes at RT

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Ku70 + Ku80 antibody to be titered up or down for optimal performance.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Détails sur Ku70 + Ku80

Antigène: Ku70 + Ku80 (KU80) (CG18801 Gene Product From Transcript CG18801-RA (KU80))

Autre désignation: Ku70 + Ku80 (KU80 Produits)

Sujet: This antibody recognizes a dimer of two proteins of 70  kDa (Ku70) and ~80  kDa (Ku80), identified as two subunits of Ku. Antbody KU729 recognizes a conformational epitope of the Ku70 + Ku80 dimer, which is destroyed during Western blotting. The Ku70 + Ku80 dimer is important for function of a 460  kDa DNA-dependent protein kinase. Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis.

ID gène: 2547

UniProt: Q6FG89