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Melanoma anticorps

Reactivité: Humain, Chien, Souris IHC (p), IF, WB Hôte: Souris Monoclonal PNL2 unconjugated
N° du produit ABIN6252231
  • Antigène Tous les produits Melanoma
    Melanoma
    Reactivité
    • 4
    • 2
    • 1
    • 1
    Humain, Chien, Souris
    Hôte
    • 4
    Souris
    Clonalité
    • 4
    Monoclonal
    Conjugué
    • 4
    Cet anticorp Melanoma est non-conjugé
    Application
    • 3
    • 3
    • 2
    • 1
    Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Western Blotting (WB)
    Purification
    Protein G affinity chromatography
    Immunogène
    Melanocyte antigen was used as the immunogen for the Melanoma antibody PNL2.
    Clone
    PNL2
    Isotype
    IgG1
  • Indications d'application
    Immunofluorescence: 0.5-1 μg/mL

    Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)

    Prediluted format: incubate for 30 min at RT (2)

    Optimal dilution of the Melanoma antibody PNL2 should be determined by the researcher.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.2 mg/mL
    Buffer
    PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    -20 °C
    Stockage commentaire
    Aliquot and Store at -20C. Avoid freez-thaw cycles.
  • Antigène
    Melanoma
    Abstract
    Melanoma Produits
    Sujet
    Anti-PNL2 is a novel monoclonal antibody, which has recently been introduced as an immunohistochemical reagent to stain melanocytes and tumors derived therefrom. The antigen recognized by PNL2 is different from Melan A and gp100. Its epitope is not destroyed by digestion with neuraminidase i.e. its epitope id not glycosylated. Anti-PNL2 may be most useful because of its high sensitivity for metastatic melanoma (87 % ), as opposed to 76 % for anti-HMB45 and 82 % for anti-MART-1. Anti-PNL2 labels intra-epidermal nevi while the dermal component of compound nevi are largely non-reactive with anti-PNL2. Antibodies against PNL2, MART-1 (Melan A) and HMB45 stain most clear cell sarcoma cells and a few cells in angio-myolipomas and lymphangioleiomyomatosis. Anti-PNL2 is a useful antibody for the identification of melanomas and clear cell sarcomas. Differential diagnosis is aided by the results from a panel of antibodies, including antibodies against HMB45, MART-1, tyrosinase, and MiTF.
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