RPA2 anticorps (pThr21)

N° du produit ABIN6255629

Détail du produit anti-RPA2 anticorps

Antigène: RPA2 (Replication Protein A2, 32kDa (RPA2))

Épitope: pThr21

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp RPA2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificité: Phospho-RPA32/RPA2 (Thr21) Antibody detects endogenous levels of RPA32/RPA2 only when phosphorylated at Threonine 21.

Homologie: Pig,Zebrafish,Bovine,Sheep,Dog,Xenopus

Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Immunogène: A synthesized peptide derived from human RPA32/RPA2 around the phosphorylation site of Thr21.

Isotype: IgG

Information d'application

Indications d'application: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at -20 °C. Stable for 12 months from date of receipt.

Date de péremption: 12 months

Détails sur RPA2

Antigène: RPA2 (Replication Protein A2, 32kDa (RPA2))

Autre désignation: RPA2 (RPA2 Produits)

Synonymes: anticorps REPA2, anticorps RPA32, anticorps 153194_at, anticorps CG9273, anticorps Dmel\\CG9273, anticorps dRPA2, anticorps rpa, anticorps zgc:109822, anticorps ik:tdsubc_2g1, anticorps xx:tdsubc_2g1, anticorps repa2, anticorps rpa32, anticorps RPA2, anticorps GB13976, anticorps LOC100230445, anticorps AA409079, anticorps AI325195, anticorps AU020965, anticorps RPA34, anticorps Rf-A2, anticorps replication protein A2, anticorps Replication protein A2, anticorps replication protein A2, 32kDa, anticorps replication protein A 32 kDa subunit, anticorps RPA2, anticorps Rpa2, anticorps rpa2, anticorps LOC725418, anticorps RPA2_2

Sujet:

Description: As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Plays also a role in base excision repair (BER) probably through interaction with UNG. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance.

Gene: RPA2

Poids moléculaire: 35kDa

ID gène: 6118

UniProt: P15927

Pathways: Telomere Maintenance, Réparation de l'ADN, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA