MED1 anticorps (pThr1457)

N° du produit ABIN6256479

Détail du produit anti-MED1 anticorps

Antigène: MED1 (Mediator Complex Subunit 1 (MED1))

Épitope: pThr1457

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MED1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificité: Phospho-TRAP220/MED1 (Thr1457) Antibody detects endogenous levels of TRAP220/MED1 only when phosphorylated at Threonine 1457.

Homologie: Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus

Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Immunogène: A synthesized peptide derived from human TRAP220/MED1 around the phosphorylation site of Thr1457.

Isotype: IgG

Information d'application

Indications d'application: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at -20 °C. Stable for 12 months from date of receipt.

Date de péremption: 12 months

Détails sur MED1

Antigène: MED1 (Mediator Complex Subunit 1 (MED1))

Autre désignation: MED1 (MED1 Produits)

Synonymes: anticorps CRSP1, anticorps CRSP200, anticorps DRIP205, anticorps DRIP230, anticorps PBP, anticorps PPARBP, anticorps PPARGBP, anticorps RB18A, anticorps TRAP220, anticorps TRIP2, anticorps CRSP-1, anticorps MED1, anticorps pbp, anticorps nbr1, anticorps crsp1, anticorps rb18a, anticorps trip2, anticorps pparbp, anticorps crsp200, anticorps drip205, anticorps drip230, anticorps ppargbp, anticorps trap220, anticorps CG7162, anticorps Dmel\\CG7162, anticorps Med1, anticorps Med1/SOP3/TRAP220, anticorps Trap220, anticorps dTRAP220, anticorps AI480703, anticorps CRSP210, anticorps Pparbp, anticorps l11Jus15, anticorps RGD1559552, anticorps mediator complex subunit 1, anticorps calcitonin-related polypeptide beta, anticorps Mediator complex subunit 1, anticorps mediator of RNA polymerase II transcription subunit 1, anticorps MED1, anticorps CALCB, anticorps med1, anticorps Med1

Sujet:

Description: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors (PubMed:10406464, PubMed:11867769, PubMed:12037571, PubMed:12218053, PubMed:12556447, PubMed:14636573, PubMed:15340084, PubMed:15471764, PubMed:15989967, PubMed:16574658, PubMed:9653119). Acts as a coactivator for GATA1-mediated transcriptional activation during erythroid differentiation of K562 erythroleukemia cells (PubMed:24245781).

Gene: MED1

Poids moléculaire: 168kDa

ID gène: 5469

UniProt: Q15648

Pathways: Nuclear Receptor Transcription Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, Chromatin Binding, Regulation of Lipid Metabolism by PPARalpha