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NONO anticorps (C-Term)

NONO Reactivité: Humain, Souris, Rat WB, IHC, ELISA, IF, ICC Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN6256820
  • Antigène Voir toutes NONO Anticorps
    NONO (Non-POU Domain Containing, Octamer-Binding (NONO))
    Épitope
    • 15
    • 13
    • 8
    • 6
    • 5
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    C-Term
    Reactivité
    • 77
    • 37
    • 33
    • 15
    • 13
    • 6
    • 5
    • 5
    • 5
    • 3
    • 3
    • 1
    Humain, Souris, Rat
    Hôte
    • 70
    • 6
    • 2
    Lapin
    Clonalité
    • 63
    • 15
    Polyclonal
    Conjugué
    • 38
    • 6
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp NONO est non-conjugé
    Application
    • 60
    • 34
    • 26
    • 15
    • 14
    • 14
    • 14
    • 8
    • 4
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)
    Specificité
    Nono Antibody detects endogenous levels of total Nono.
    Homologie
    Pig,Bovine,Horse,Sheep,Rabbit,Dog
    Purification
    The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
    Immunogène
    A synthesized peptide derived from human Nono, corresponding to a region within C-terminal amino acids.
    Isotype
    IgG
    Top Product
    Discover our top product NONO Anticorps primaire
  • Indications d'application
    WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    -20 °C
    Stockage commentaire
    Store at -20 °C. Stable for 12 months from date of receipt.
    Date de péremption
    12 months
  • Antigène
    NONO (Non-POU Domain Containing, Octamer-Binding (NONO))
    Autre désignation
    NONO (NONO Produits)
    Synonymes
    anticorps NMT55, anticorps NRB54, anticorps P54, anticorps P54NRB, anticorps nmt55, anticorps nrb54, anticorps p54nrb, anticorps xp54nrb, anticorps AA407051, anticorps AV149256, anticorps nonA, anticorps non-POU domain containing octamer binding, anticorps non-POU domain containing, octamer-binding, anticorps non-POU domain containing, octamer binding L homeolog, anticorps non-POU-domain-containing, octamer binding protein, anticorps NONO, anticorps Nono, anticorps nono.L
    Sujet

    Description: DNA- and RNA binding protein, involved in several nuclear processes. Binds the conventional octamer sequence in double-stranded DNA. Also binds single-stranded DNA and RNA at a site independent of the duplex site. Involved in pre-mRNA splicing, probably as a heterodimer with SFPQ. Interacts with U5 snRNA, probably by binding to a purine-rich sequence located on the 3' side of U5 snRNA stem 1b. Together with PSPC1, required for the formation of nuclear paraspeckles. The SFPQ-NONO heteromer associated with MATR3 may play a role in nuclear retention of defective RNAs. The SFPQ-NONO heteromer may be involved in DNA unwinding by modulating the function of topoisomerase I/TOP1. The SFPQ-NONO heteromer may be involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination and may stabilize paired DNA ends. In vitro, the complex strongly stimulates DNA end joining, binds directly to the DNA substrates and cooperates with the Ku70/G22P1-Ku80/XRCC5 (Ku) dimer to establish a functional preligation complex. NONO is involved in transcriptional regulation. The SFPQ-NONO-NR5A1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional activity. NONO binds to an enhancer element in long terminal repeats of endogenous intracisternal A particles (IAPs) and activates transcription. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer. Important for the functional organization of GABAergic synapses. Plays a specific and important role in the regulation of synaptic RNAs and GPHN/gephyrin scaffold structure, through the regulation of GABRA2 transcript. Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway (PubMed:28712728).

    Gene: NONO

    Poids moléculaire
    54kDa
    ID gène
    4841
    UniProt
    Q15233
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