RNF138 anticorps (Internal Region)

N° du produit ABIN6257503

Détail du produit anti-RNF138 anticorps

Antigène: RNF138 (Ring Finger Protein 138 (RNF138))

Épitope: Internal Region

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp RNF138 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

Specificité: RNF138 Antibody detects endogenous levels of total RNF138.

Homologie: Pig,Bovine,Horse,Sheep,Rabbit,Dog,Chicken

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogène: A synthesized peptide derived from human RNF138, corresponding to a region within the internal amino acids.

Isotype: IgG

Information d'application

Indications d'application: WB 1:500-1:1000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at -20 °C. Stable for 12 months from date of receipt.

Date de péremption: 12 months

Détails sur RNF138

Antigène: RNF138 (Ring Finger Protein 138 (RNF138))

Autre désignation: RNF138 (RNF138 Produits)

Synonymes: anticorps 2410015A17Rik, anticorps 2810480D20Rik, anticorps STRIN, anticorps Trif, anticorps Trif-d, anticorps HSD-4, anticorps NARF, anticorps hNARF, anticorps Rsd4, anticorps narf, anticorps ring finger protein 138, anticorps ring finger protein 138 S homeolog, anticorps RNF138, anticorps Rnf138, anticorps rnf138.S

Sujet:

Description: E3 ubiquitin-protein ligase involved in DNA damage response by promoting DNA resection and homologous recombination (PubMed:26502055, PubMed:26502057). Recruited to sites of double-strand breaks following DNA damage and specifically promotes double-strand break repair via homologous recombination (PubMed:26502055, PubMed:26502057). Two different, non-exclusive, mechanisms have been proposed. According to a report, regulates the choice of double-strand break repair by favoring homologous recombination over non-homologous end joining (NHEJ): acts by mediating ubiquitination of XRCC5/Ku80, leading to remove the Ku complex from DNA breaks, thereby promoting homologous recombination (PubMed:26502055). According to another report, cooperates with UBE2Ds E2 ubiquitin ligases (UBE2D1, UBE2D2, UBE2D3 or UBE2D4) to promote homologous recombination by mediating ubiquitination of RBBP8/CtIP (PubMed:26502057). Together with NLK, involved in the ubiquitination and degradation of TCF/LEF (PubMed:16714285). Also exhibits auto-ubiquitination activity in combination with UBE2K (PubMed:16714285). May act as a negative regulator in the Wnt/beta-catenin-mediated signaling pathway (PubMed:16714285).

Gene: RNF138

Poids moléculaire: 28 kDa

ID gène: 51444

UniProt: Q8WVD3