NCBP1 anticorps (N-Term)

N° du produit ABIN6257658

Détail du produit anti-NCBP1 anticorps

Antigène: NCBP1 (Nuclear Cap Binding Protein Subunit 1, 80kDa (NCBP1))

Épitope: N-Term

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp NCBP1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificité: NCBP1 Antibody detects endogenous levels of total NCBP1.

Homologie: Pig,Bovine,Sheep,Rabbit,Dog,Chicken,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogène: A synthesized peptide derived from human NCBP1, corresponding to a region within N-terminal amino acids.

Isotype: IgG

Information d'application

Indications d'application: WB 1:500-1:1000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at -20 °C. Stable for 12 months from date of receipt.

Date de péremption: 12 months

Détails sur NCBP1

Antigène: NCBP1 (Nuclear Cap Binding Protein Subunit 1, 80kDa (NCBP1))

Autre désignation: NCBP1 (NCBP1 Produits)

Synonymes: anticorps CBP80, anticorps CG7035, anticorps Dmel\\CG7035, anticorps EG:84H4.3, anticorps ncbp, anticorps sto1, anticorps cbp80, anticorps AU014645, anticorps AW538051, anticorps NCBP, anticorps Sto1, anticorps ncbp1, anticorps ABA HYPERSENSITIVE 1, anticorps ATCBP80, anticorps CAP-BINDING PROTEIN 80, anticorps ENS, anticorps ENSALADA, anticorps NUCLEAR CAP-BINDING PROTEIN CBP80, anticorps T10F5.8, anticorps T10F5_8, anticorps cap binding protein 80, anticorps nuclear cap binding protein subunit 1, anticorps nuclear cap binding protein subunit 1 S homeolog, anticorps nuclear cap binding protein subunit 1, 80kDa, anticorps nuclear cap binding protein subunit 1 L homeolog, anticorps ARM repeat superfamily protein, anticorps Nuclear cap-binding protein subunit 1-A, anticorps Nuclear cap-binding protein subunit 1, anticorps Cbp80, anticorps ncbp1, anticorps ncbp1.S, anticorps NCBP1, anticorps Ncbp1, anticorps ncbp1.L, anticorps ABH1, anticorps nbp1a, anticorps ncbp-1

Sujet:

Description: Component of the cap-binding complex (CBC), which binds cotranscriptionally to the 5'-cap of pre-mRNAs and is involved in various processes such as pre-mRNA splicing, translation regulation, nonsense-mediated mRNA decay, RNA-mediated gene silencing (RNAi) by microRNAs (miRNAs) and mRNA export. The CBC complex is involved in mRNA export from the nucleus via its interaction with ALYREF/THOC4/ALY, leading to the recruitment of the mRNA export machinery to the 5'-end of mRNA and to mRNA export in a 5' to 3' direction through the nuclear pore. The CBC complex is also involved in mediating U snRNA and intronless mRNAs export from the nucleus. The CBC complex is essential for a pioneer round of mRNA translation, before steady state translation when the CBC complex is replaced by cytoplasmic cap-binding protein eIF4E. The pioneer round of mRNA translation mediated by the CBC complex plays a central role in nonsense-mediated mRNA decay (NMD), NMD only taking place in mRNAs bound to the CBC complex, but not on eIF4E-bound mRNAs. The CBC complex enhances NMD in mRNAs containing at least one exon-junction complex (EJC) via its interaction with UPF1, promoting the interaction between UPF1 and UPF2. The CBC complex is also involved in 'failsafe' NMD, which is independent of the EJC complex, while it does not participate in Staufen-mediated mRNA decay (SMD). During cell proliferation, the CBC complex is also involved in microRNAs (miRNAs) biogenesis via its interaction with SRRT/ARS2 and is required for miRNA-mediated RNA interference. The CBC complex also acts as a negative regulator of PARN, thereby acting as an inhibitor of mRNA deadenylation. In the CBC complex, NCBP1/CBP80 does not bind directly capped RNAs (m7GpppG-capped RNA) but is required to stabilize the movement of the N-terminal loop of NCBP2/CBP20 and lock the CBC into a high affinity cap-binding state with the cap structure. Associates with NCBP3 to form an alternative cap-binding complex (CBC) which plays a key role in mRNA export and is particularly important in cellular stress situations such as virus infections. The conventional CBC with NCBP2 binds both small nuclear RNA (snRNA) and messenger (mRNA) and is involved in their export from the nucleus whereas the alternative CBC with NCBP3 does not bind snRNA and associates only with mRNA thereby playing a role only in mRNA export. NCBP1/CBP80 is required for cell growth and viability (PubMed:26382858).

Gene: NCBP1

Poids moléculaire: 80 kDa

ID gène: 4686

UniProt: Q09161

Pathways: Ribonucleoprotein Complex Subunit Organization, Photoperiodism, Methionine Biosynthetic Process