SRPK1 anticorps (C-Term)

N° du produit ABIN6259197

Détail du produit anti-SRPK1 anticorps

Antigène: SRPK1 (SRSF Protein Kinase 1 (SRPK1))

Épitope: C-Term

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SRPK1 est non-conjugé

Application: Western Blotting (WB), ELISA

Specificité: SRPK1 Antibody detects endogenous levels of total SRPK1.

Homologie: Pig,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogène: A synthesized peptide derived from human SRPK1, corresponding to a region within C-terminal amino acids.

Isotype: IgG

Information d'application

Indications d'application: WB 1:500-1:1000, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at -20 °C. Stable for 12 months from date of receipt.

Date de péremption: 12 months

Détails sur SRPK1

Antigène: SRPK1 (SRSF Protein Kinase 1 (SRPK1))

Autre désignation: SRPK1 (SRPK1 Produits)

Synonymes: anticorps SFRSK1, anticorps AU017960, anticorps BEST:CK01209, anticorps CG11489, anticorps CG9085, anticorps CK01209, anticorps Dmel\\CG11489, anticorps SRPK, anticorps SRPK1, anticorps Srpk79D, anticorps atc, anticorps srpk1, anticorps zgc:63789, anticorps wu:fc06h03, anticorps zgc:158800, anticorps sfrsk1, anticorps SRSF protein kinase 1, anticorps serine/arginine-rich protein specific kinase 1, anticorps Serine-arginine protein kinase at 79D, anticorps SRSF protein kinase 1a, anticorps SRSF protein kinase 1b, anticorps SRSF protein kinase 1 S homeolog, anticorps SRPK1, anticorps Srpk1, anticorps Srpk79D, anticorps srpk1a, anticorps srpk1b, anticorps srpk1.S

Sujet:

Description: Serine/arginine-rich protein-specific kinase which specifically phosphorylates its substrates at serine residues located in regions rich in arginine/serine dipeptides, known as RS domains and is involved in the phosphorylation of SR splicing factors and the regulation of splicing. Plays a central role in the regulatory network for splicing, controlling the intranuclear distribution of splicing factors in interphase cells and the reorganization of nuclear speckles during mitosis. Can influence additional steps of mRNA maturation, as well as other cellular activities, such as chromatin reorganization in somatic and sperm cells and cell cycle progression. Isoform 2 phosphorylates SFRS2, ZRSR2, LBR and PRM1. Isoform 2 phosphorylates SRSF1 using a directional (C-terminal to N-terminal) and a dual-track mechanism incorporating both processive phosphorylation (in which the kinase stays attached to the substrate after each round of phosphorylation) and distributive phosphorylation steps (in which the kinase and substrate dissociate after each phosphorylation event). The RS domain of SRSF1 binds first to a docking groove in the large lobe of the kinase domain of SRPK1. This induces certain structural changes in SRPK1 and/or RRM2 domain of SRSF1, allowing RRM2 to bind the kinase and initiate phosphorylation. The cycles continue for several phosphorylation steps in a processive manner (steps 1-8) until the last few phosphorylation steps (approximately steps 9-12). During that time, a mechanical stress induces the unfolding of the beta-4 motif in RRM2, which then docks at the docking groove of SRPK1. This also signals RRM2 to begin to dissociate, which facilitates SRSF1 dissociation after phosphorylation is completed. Isoform 2 can mediate hepatitis B virus (HBV) core protein phosphorylation. It plays a negative role in the regulation of HBV replication through a mechanism not involving the phosphorylation of the core protein but by reducing the packaging efficiency of the pregenomic RNA (pgRNA) without affecting the formation of the viral core particles. Isoform 1 and isoform 2 can induce splicing of exon 10 in MAPT/TAU. The ratio of isoform 1/isoform 2 plays a decisive role in determining cell fate in K-562 leukaemic cell line: isoform 2 favors proliferation where as isoform 1 favors differentiation.

Gene: SRPK1

Poids moléculaire: 95 kDa

ID gène: 6732

UniProt: Q96SB4

Pathways: Toll-Like Receptors Cascades