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TOP3B anticorps (C-Term)

TOP3B Reactivité: Humain, Souris WB, ELISA, IHC, ICC, IF Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN6259219
  • Antigène Voir toutes TOP3B Anticorps
    TOP3B (Topoisomerase (DNA) III beta (TOP3B))
    Épitope
    • 8
    • 7
    • 6
    • 4
    • 3
    • 1
    • 1
    • 1
    • 1
    C-Term
    Reactivité
    • 34
    • 20
    • 3
    • 1
    • 1
    • 1
    Humain, Souris
    Hôte
    • 31
    • 4
    Lapin
    Clonalité
    • 32
    • 3
    Polyclonal
    Conjugué
    • 18
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp TOP3B est non-conjugé
    Application
    • 28
    • 17
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)
    Specificité
    TOP3B Antibody detects endogenous levels of total TOP3B.
    Homologie
    Rabbit
    Purification
    The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
    Immunogène
    A synthesized peptide derived from human TOP3B, corresponding to a region within C-terminal amino acids.
    Isotype
    IgG
    Top Product
    Discover our top product TOP3B Anticorps primaire
  • Indications d'application
    WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    -20 °C
    Stockage commentaire
    Store at -20 °C. Stable for 12 months from date of receipt.
    Date de péremption
    12 months
  • Antigène
    TOP3B (Topoisomerase (DNA) III beta (TOP3B))
    Autre désignation
    TOP3B (TOP3B Produits)
    Synonymes
    anticorps TOP3B1, anticorps top3b, anticorps DNA topoisomerase III beta, anticorps topoisomerase (DNA) III beta, anticorps DNA topoisomerase III beta L homeolog, anticorps TOP3B, anticorps Top3b, anticorps top3b.L
    Sujet

    Description: Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Possesses negatively supercoiled DNA relaxing activity.

    Gene: TOP3B

    Poids moléculaire
    96 kDa
    ID gène
    8940
    UniProt
    O95985
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