AGO2 anticorps (N-Term)

N° du produit ABIN6259825

Détail du produit anti-AGO2 anticorps

Antigène: AGO2 (Argonaute 2 (AGO2))

Épitope: N-Term

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp AGO2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Specificité: AGO2 Antibody detects endogenous levels of total AGO2.

Homologie: Pig,Zebrafish,Bovine,Horse,Sheep,Rabbit,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogène: A synthesized peptide derived from human AGO2, corresponding to a region within N-terminal amino acids.

Isotype: IgG

Information d'application

Indications d'application: WB 1:500-1:2000, IHC 1:50-1:200

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at -20 °C. Stable for 12 months from date of receipt.

Date de péremption: 12 months

Détails sur AGO2

Antigène: AGO2 (Argonaute 2 (AGO2))

Autre désignation: AGO2 (AGO2 Produits)

Synonymes: anticorps EIF2C2, anticorps T19E23.7, anticorps T19E23_7, anticorps argonaute 2, anticorps Q10, anticorps Eif2c2, anticorps Argonaute2, anticorps eif2c1, anticorps eif2c2, anticorps 1110029L17Rik, anticorps 2310051F07Rik, anticorps AI225898, anticorps AL022874, anticorps AW546247, anticorps ENSMUSG00000072493, anticorps Gerp95, anticorps Gm10365, anticorps mKIAA4215, anticorps AGO2, anticorps AG02, anticorps AGO 2, anticorps Ago-2, anticorps Ago2, anticorps CG13452, anticorps CG7439, anticorps Dm Ago2, anticorps Dmel\\CG7439, anticorps ago, anticorps ago-2, anticorps ago2, anticorps dAGO2, anticorps dAgo2, anticorps eIF2C2, anticorps argonaute 2, RISC catalytic component, anticorps Argonaute family protein, anticorps argonaute 2, RISC catalytic component L homeolog, anticorps argonaute RISC catalytic subunit 2, anticorps argonaute RISC catalytic component 2, anticorps Argonaute 2, anticorps AGO2, anticorps Ago2, anticorps ago2.L, anticorps ago2

Sujet:

Description: Required for RNA-mediated gene silencing (RNAi) by the RNA-induced silencing complex (RISC). The 'minimal RISC' appears to include AGO2 bound to a short guide RNA such as a microRNA (miRNA) or short interfering RNA (siRNA). These guide RNAs direct RISC to complementary mRNAs that are targets for RISC-mediated gene silencing. The precise mechanism of gene silencing depends on the degree of complementarity between the miRNA or siRNA and its target. Binding of RISC to a perfectly complementary mRNA generally results in silencing due to endonucleolytic cleavage of the mRNA specifically by AGO2. Binding of RISC to a partially complementary mRNA results in silencing through inhibition of translation, and this is independent of endonuclease activity. May inhibit translation initiation by binding to the 7-methylguanosine cap, thereby preventing the recruitment of the translation initiation factor eIF4-E. May also inhibit translation initiation via interaction with EIF6, which itself binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit. The inhibition of translational initiation leads to the accumulation of the affected mRNA in cytoplasmic processing bodies (P-bodies), where mRNA degradation may subsequently occur. In some cases RISC-mediated translational repression is also observed for miRNAs that perfectly match the 3' untranslated region (3'-UTR). Can also up-regulate the translation of specific mRNAs under certain growth conditions. Binds to the AU element of the 3'-UTR of the TNF (TNF-alpha) mRNA and up-regulates translation under conditions of serum starvation. Also required for transcriptional gene silencing (TGS), in which short RNAs known as antigene RNAs or agRNAs direct the transcriptional repression of complementary promoter regions.

Gene: AGO2

Poids moléculaire: 90-95 kDa

ID gène: 27161

UniProt: Q9UKV8

Pathways: Fc-epsilon Receptor Signaling Pathway, Regulatory RNA Pathways, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Ribonucleoprotein Complex Subunit Organization