DAP Kinase 1 anticorps (Internal Region)

N° du produit ABIN6261221

Détail du produit anti-DAP Kinase 1 anticorps

Antigène: DAP Kinase 1 (DAPK1) (Death-Associated Protein Kinase 1 (DAPK1))

Épitope: Internal Region

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp DAP Kinase 1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Specificité: DAPK1 Antibody detects endogenous levels of total DAPK1.

Homologie: Pig,Zebrafish,Bovine,Horse,Rabbit,Dog,Chicken,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogène: A synthesized peptide derived from human DAPK1, corresponding to a region within the internal amino acids.

Isotype: IgG

Information d'application

Indications d'application: WB 1:1000-3000, IHC 1:200, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at -20 °C. Stable for 12 months from date of receipt.

Date de péremption: 12 months

Détails sur DAP Kinase 1

Antigène: DAP Kinase 1 (DAPK1) (Death-Associated Protein Kinase 1 (DAPK1))

Autre désignation: DAPK1 (DAPK1 Produits)

Synonymes: anticorps DAPK1, anticorps MGC81366, anticorps si:ch211-66i11.1, anticorps wu:fj09c03, anticorps dapk, anticorps MGC83745, anticorps 2310039H24Rik, anticorps 2810425C21Rik, anticorps AI642003, anticorps D13Ucla1, anticorps DAP-Kinase, anticorps DAPK, anticorps death-associated protein kinase 1, anticorps death associated protein kinase 1 L homeolog, anticorps death associated protein kinase 1, anticorps death associated protein kinase 1 S homeolog, anticorps LOC427465, anticorps dapk1.L, anticorps DAPK1, anticorps dapk1, anticorps dapk1.S, anticorps Dapk1

Sujet:

Description: Calcium/calmodulin-dependent serine/threonine kinase involved in multiple cellular signaling pathways that trigger cell survival, apoptosis, and autophagy. Regulates both type I apoptotic and type II autophagic cell deaths signal, depending on the cellular setting. The former is caspase-dependent, while the latter is caspase-independent and is characterized by the accumulation of autophagic vesicles. Phosphorylates PIN1 resulting in inhibition of its catalytic activity, nuclear localization, and cellular function. Phosphorylates TPM1, enhancing stress fiber formation in endothelial cells. Phosphorylates STX1A and significantly decreases its binding to STXBP1. Phosphorylates PRKD1 and regulates JNK signaling by binding and activating PRKD1 under oxidative stress. Phosphorylates BECN1, reducing its interaction with BCL2 and BCL2L1 and promoting the induction of autophagy. Phosphorylates TSC2, disrupting the TSC1-TSC2 complex and stimulating mTORC1 activity in a growth factor-dependent pathway. Phosphorylates RPS6, MYL9 and DAPK3. Acts as a signaling amplifier of NMDA receptors at extrasynaptic sites for mediating brain damage in stroke. Cerebral ischemia recruits DAPK1 into the NMDA receptor complex and it phosphorylates GRINB at Ser-1303 inducing injurious Ca2+ influx through NMDA receptor channels, resulting in an irreversible neuronal death. Required together with DAPK3 for phosphorylation of RPL13A upon interferon-gamma activation which is causing RPL13A involvement in transcript-selective translation inhibition.

Gene: DAPK1

Poids moléculaire: 160kDa

ID gène: 1612

UniProt: P53355

Pathways: Signalisation MAPK, Interferon-gamma Pathway