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DGCR8 anticorps (C-Term)

DGCR8 Reactivité: Humain, Souris, Rat WB, ELISA, IF, ICC Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN6261282
  • Antigène Voir toutes DGCR8 Anticorps
    DGCR8 (DiGeorge Syndrome Critical Region Gene 8 (DGCR8))
    Épitope
    • 8
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term
    Reactivité
    • 41
    • 15
    • 13
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Humain, Souris, Rat
    Hôte
    • 34
    • 6
    • 1
    Lapin
    Clonalité
    • 29
    • 12
    Polyclonal
    Conjugué
    • 29
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp DGCR8 est non-conjugé
    Application
    • 30
    • 17
    • 9
    • 8
    • 6
    • 5
    • 3
    • 2
    • 1
    Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)
    Specificité
    DGCR8 Antibody detects endogenous levels of total DGCR8.
    Homologie
    Pig,Zebrafish,Bovine,Horse,Sheep,Rabbit,Dog,Xenopus
    Purification
    The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
    Immunogène
    A synthesized peptide derived from human DGCR8, corresponding to a region within C-terminal amino acids.
    Isotype
    IgG
    Top Product
    Discover our top product DGCR8 Anticorps primaire
  • Indications d'application
    WB 1:1000-3000, IF/ICC, ELISA(peptide) 1:20000-1:40000
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    -20 °C
    Stockage commentaire
    Store at -20 °C. Stable for 12 months from date of receipt.
    Date de péremption
    12 months
  • Antigène
    DGCR8 (DiGeorge Syndrome Critical Region Gene 8 (DGCR8))
    Autre désignation
    DGCR8 (DGCR8 Produits)
    Synonymes
    anticorps MGC78846, anticorps DGCR8, anticorps gy1, anticorps dgcrk6, anticorps C22orf12, anticorps DGCRK6, anticorps Gy1, anticorps pasha, anticorps D16H22S1742E, anticorps D16H22S788E, anticorps D16Wis2, anticorps N41, anticorps Vo59c07, anticorps si:ch211-106a19.4, anticorps wu:fc23f08, anticorps wu:fc38f06, anticorps DGCR8 microprocessor complex subunit L homeolog, anticorps DiGeorge syndrome critical region gene 8, anticorps DGCR8 microprocessor complex subunit, anticorps DGCR8, microprocessor complex subunit, anticorps microRNA 3618, anticorps dgcr8.L, anticorps DGCR8, anticorps dgcr8, anticorps Dgcr8, anticorps MIR3618
    Sujet

    Description: Component of the microprocessor complex that acts as a RNA- and heme-binding protein that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DGCR8 function as a molecular anchor necessary for the recognition of pri-miRNA at dsRNA-ssRNA junction and directs DROSHA to cleave 11 bp away form the junction to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs (PubMed:26027739, PubMed:26748718). The heme-bound DGCR8 dimer binds pri-miRNAs as a cooperative trimer (of dimers) and is active in triggering pri-miRNA cleavage, whereas the heme-free DGCR8 monomer binds pri-miRNAs as a dimer and is much less active. Both double-stranded and single-stranded regions of a pri-miRNA are required for its binding (PubMed:15531877, PubMed:15574589, PubMed:15589161, PubMed:16751099, PubMed:16906129, PubMed:16963499, PubMed:17159994). Specifically recognizes and binds N6-methyladenosine (m6A)-containing pri-miRNAs, a modification required for pri-miRNAs processing (PubMed:25799998). Involved in the silencing of embryonic stem cell self-renewal (By similarity).

    Gene: DGCR8

    Poids moléculaire
    100kDa
    ID gène
    54487
    UniProt
    Q8WYQ5
    Pathways
    Regulatory RNA Pathways
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