DGCR8 anticorps (C-Term)
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- Antigène Voir toutes DGCR8 Anticorps
- DGCR8 (DiGeorge Syndrome Critical Region Gene 8 (DGCR8))
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Épitope
- C-Term
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Reactivité
- Humain, Souris, Rat
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp DGCR8 est non-conjugé
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Application
- Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)
- Specificité
- DGCR8 Antibody detects endogenous levels of total DGCR8.
- Homologie
- Pig,Zebrafish,Bovine,Horse,Sheep,Rabbit,Dog,Xenopus
- Purification
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
- Immunogène
- A synthesized peptide derived from human DGCR8, corresponding to a region within C-terminal amino acids.
- Isotype
- IgG
- Top Product
- Discover our top product DGCR8 Anticorps primaire
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- Indications d'application
- WB 1:1000-3000, IF/ICC, ELISA(peptide) 1:20000-1:40000
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- -20 °C
- Stockage commentaire
- Store at -20 °C. Stable for 12 months from date of receipt.
- Date de péremption
- 12 months
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- Antigène
- DGCR8 (DiGeorge Syndrome Critical Region Gene 8 (DGCR8))
- Autre désignation
- DGCR8 (DGCR8 Produits)
- Synonymes
- anticorps MGC78846, anticorps DGCR8, anticorps gy1, anticorps dgcrk6, anticorps C22orf12, anticorps DGCRK6, anticorps Gy1, anticorps pasha, anticorps D16H22S1742E, anticorps D16H22S788E, anticorps D16Wis2, anticorps N41, anticorps Vo59c07, anticorps si:ch211-106a19.4, anticorps wu:fc23f08, anticorps wu:fc38f06, anticorps DGCR8 microprocessor complex subunit L homeolog, anticorps DiGeorge syndrome critical region gene 8, anticorps DGCR8 microprocessor complex subunit, anticorps DGCR8, microprocessor complex subunit, anticorps microRNA 3618, anticorps dgcr8.L, anticorps DGCR8, anticorps dgcr8, anticorps Dgcr8, anticorps MIR3618
- Sujet
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Description: Component of the microprocessor complex that acts as a RNA- and heme-binding protein that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DGCR8 function as a molecular anchor necessary for the recognition of pri-miRNA at dsRNA-ssRNA junction and directs DROSHA to cleave 11 bp away form the junction to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs (PubMed:26027739, PubMed:26748718). The heme-bound DGCR8 dimer binds pri-miRNAs as a cooperative trimer (of dimers) and is active in triggering pri-miRNA cleavage, whereas the heme-free DGCR8 monomer binds pri-miRNAs as a dimer and is much less active. Both double-stranded and single-stranded regions of a pri-miRNA are required for its binding (PubMed:15531877, PubMed:15574589, PubMed:15589161, PubMed:16751099, PubMed:16906129, PubMed:16963499, PubMed:17159994). Specifically recognizes and binds N6-methyladenosine (m6A)-containing pri-miRNAs, a modification required for pri-miRNAs processing (PubMed:25799998). Involved in the silencing of embryonic stem cell self-renewal (By similarity).
Gene: DGCR8
- Poids moléculaire
- 100kDa
- ID gène
- 54487
- UniProt
- Q8WYQ5
- Pathways
- Regulatory RNA Pathways
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