DNA2 anticorps (Internal Region)

N° du produit ABIN6261326

Détail du produit anti-DNA2 anticorps

Antigène: DNA2 (DNA Replication Helicase 2 Homolog (DNA2))

Épitope: Internal Region

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp DNA2 est non-conjugé

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificité: DNA2L Antibody detects endogenous levels of total DNA2L.

Homologie: Pig,Bovine,Horse,Sheep,Rabbit,Dog

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogène: A synthesized peptide derived from human DNA2L, corresponding to a region within the internal amino acids.

Isotype: IgG

Information d'application

Indications d'application: WB 1:1000-3000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at -20 °C. Stable for 12 months from date of receipt.

Date de péremption: 12 months

Détails sur DNA2

Antigène: DNA2 (DNA Replication Helicase 2 Homolog (DNA2))

Autre désignation: DNA2 (DNA2 Produits)

Synonymes: anticorps DNA2L, anticorps Dna2l, anticorps E130315B21Rik, anticorps PEOA6, anticorps hDNA2, anticorps XDna2, anticorps dna2-A, anticorps DNA replication helicase/nuclease 2, anticorps DNA replication helicase/nuclease 2 S homeolog, anticorps DNA2, anticorps Dna2, anticorps dna2.S

Sujet:

Description: Key enzyme involved in DNA replication and DNA repair in nucleus and mitochondrion. Involved in Okazaki fragments processing by cleaving long flaps that escape FEN1: flaps that are longer than 27 nucleotides are coated by replication protein A complex (RPA), leading to recruit DNA2 which cleaves the flap until it is too short to bind RPA and becomes a substrate for FEN1. Also involved in 5'-end resection of DNA during double-strand break (DSB) repair: recruited by BLM and mediates the cleavage of 5'-ssDNA, while the 3'-ssDNA cleavage is prevented by the presence of RPA. Also involved in DNA replication checkpoint independently of Okazaki fragments processing. Possesses different enzymatic activities, such as single-stranded DNA (ssDNA)-dependent ATPase, 5'-3' helicase and endonuclease activities. While the ATPase and endonuclease activities are well-defined and play a key role in Okazaki fragments processing and DSB repair, the 5'-3' DNA helicase activity is subject to debate. According to various reports, the helicase activity is weak and its function remains largely unclear. Helicase activity may promote the motion of DNA2 on the flap, helping the nuclease function.

Gene: DNA2

Poids moléculaire: 120 kDa

ID gène: 1763

UniProt: P51530

Pathways: Telomere Maintenance, Réparation de l'ADN, DNA Replication, Synthesis of DNA