MEK1 anticorps (Internal Region)

N° du produit ABIN6269316

Détail du produit anti-MEK1 anticorps

Antigène: MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))

Épitope: Internal Region

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MEK1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificité: MEK1/2 Antibody detects endogenous levels of total MEK1/2.

Homologie: Pig,Zebrafish,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogène: A synthesized peptide derived from human MEK1/2, corresponding to a region within the internal amino acids.

Isotype: IgG

Information d'application

Indications d'application: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at -20 °C. Stable for 12 months from date of receipt.

Date de péremption: 12 months

Références pour anticorps anti-MEK1 (ABIN6269316)

Wu, Zhu, Yin, Kang, Cao, Tian, Zhang, Lu, Liu: "Traditional Chinese Medicine CFF-1 induced cell growth inhibition, autophagy, and apoptosis via inhibiting EGFR-related pathways in prostate cancer." dans: Cancer medicine, Vol. 7, Issue 4, pp. 1546-1559, (2018) (PubMed).

Wu, Zhu, Zhang, Yin, Kang, Cao, Tian, Lu, Liu: "EGFR‑associated pathways involved in traditional Chinese medicine (TCM)‑1‑induced cell growth inhibition, autophagy and apoptosis in prostate cancer." dans: Molecular medicine reports, Vol. 17, Issue 6, pp. 7875-7885, (2018) (PubMed).

Détails sur MEK1

Antigène: MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))

Autre désignation: MAP2K1,MAP2K2 (MAP2K1 Produits)

Synonymes: anticorps Mek1, anticorps CFC3, anticorps MAPKK1, anticorps MEK1, anticorps MKK1, anticorps PRKMK1, anticorps MEKK1, anticorps Prkmk1, anticorps mek-2, anticorps si:ch211-242m18.2, anticorps wu:fj56a12, anticorps wu:fj61b01, anticorps zgc:56557, anticorps ATMEK1, anticorps F20B18.180, anticorps F20B18_180, anticorps MAP kinase/ ERK kinase 1, anticorps MITOGEN ACTIVATED PROTEIN KINASE KINASE, anticorps MITOGEN ACTIVATED PROTEIN KINASE KINASE 1, anticorps NMAPKK, anticorps MEK, anticorps mitogen activated protein kinase kinase 1, anticorps mitogen-activated protein kinase kinase 1, anticorps mitogen-activated protein kinase kinase 1 L homeolog, anticorps MAP kinase/ ERK kinase 1, anticorps Map2k1, anticorps MAP2K1, anticorps map2k1.L, anticorps map2k1, anticorps MEK1

Sujet:

Description: Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Binding of extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation. RAF1 then further activates the dual-specificity protein kinases MAP2K1/MEK1 and MAP2K2/MEK2. Both MAP2K1/MEK1 and MAP2K2/MEK2 function specifically in the MAPK/ERK cascade, and catalyze the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in the extracellular signal-regulated kinases MAPK3/ERK1 and MAPK1/ERK2, leading to their activation and further transduction of the signal within the MAPK/ERK cascade. Depending on the cellular context, this pathway mediates diverse biological functions such as cell growth, adhesion, survival and differentiation, predominantly through the regulation of transcription, metabolism and cytoskeletal rearrangements. One target of the MAPK/ERK cascade is peroxisome proliferator-activated receptor gamma (PPARG), a nuclear receptor that promotes differentiation and apoptosis. MAP2K1/MEK1 has been shown to export PPARG from the nucleus. The MAPK/ERK cascade is also involved in the regulation of endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC), as well as in the fragmentation of the Golgi apparatus during mitosis.

Gene: MAP2K1

Poids moléculaire: 45kDa

ID gène: 5604, 5605

UniProt: Q02750, P36507

Pathways: Signalisation MAPK, Signalisation RTK, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling