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Nemo-Like Kinase anticorps (N-Term)

NLK Reactivité: Humain, Souris, Rat WB, ELISA, IHC, IF, ICC Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN6263656
  • Antigène Voir toutes Nemo-Like Kinase (NLK) Anticorps
    Nemo-Like Kinase (NLK)
    Épitope
    • 15
    • 15
    • 8
    • 8
    • 8
    • 7
    • 6
    • 6
    • 6
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    N-Term
    Reactivité
    • 81
    • 34
    • 11
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain, Souris, Rat
    Hôte
    • 82
    • 4
    Lapin
    Clonalité
    • 83
    • 3
    Polyclonal
    Conjugué
    • 26
    • 8
    • 7
    • 7
    • 5
    • 5
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp Nemo-Like Kinase est non-conjugé
    Application
    • 78
    • 40
    • 26
    • 26
    • 23
    • 15
    • 13
    • 11
    • 9
    • 6
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
    Specificité
    NLK Antibody detects endogenous levels of total NLK.
    Homologie
    Pig,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus
    Purification
    The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
    Immunogène
    A synthesized peptide derived from human NLK, corresponding to a region within N-terminal amino acids.
    Isotype
    IgG
    Top Product
    Discover our top product NLK Anticorps primaire
  • Indications d'application
    WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    -20 °C
    Stockage commentaire
    Store at -20 °C. Stable for 12 months from date of receipt.
    Date de péremption
    12 months
  • Antigène
    Nemo-Like Kinase (NLK)
    Autre désignation
    NLK (NLK Produits)
    Synonymes
    anticorps Amf, anticorps Gpi1, anticorps AI194375, anticorps nlk, anticorps nlkb, anticorps zgc:111875, anticorps Xnlk, anticorps nlk1, anticorps nlk2, anticorps NLK, anticorps nemo-like, anticorps RGD1561602, anticorps glucose-6-phosphate isomerase, anticorps nemo like kinase, anticorps nemo-like kinase, type 1, anticorps nemo-like kinase L homeolog, anticorps Nemo-Like kinase, anticorps nemo-like kinase, anticorps Gpi, anticorps NLK, anticorps Nlk, anticorps nlk1, anticorps nlk.L, anticorps nlk, anticorps nemo-like
    Sujet

    Description: Serine/threonine-protein kinase that regulates a number of transcription factors with key roles in cell fate determination. Positive effector of the non-canonical Wnt signaling pathway, acting downstream of WNT5A, MAP3K7/TAK1 and HIPK2. Activation of this pathway causes binding to and phosphorylation of the histone methyltransferase SETDB1. The NLK-SETDB1 complex subsequently interacts with PPARG, leading to methylation of PPARG target promoters at histone H3K9 and transcriptional silencing. The resulting loss of PPARG target gene transcription inhibits adipogenesis and promotes osteoblastogenesis in mesenchymal stem cells (MSCs). Negative regulator of the canonical Wnt/beta-catenin signaling pathway. Binds to and phosphorylates TCF7L2/TCF4 and LEF1, promoting the dissociation of the TCF7L2/LEF1/beta-catenin complex from DNA, as well as the ubiquitination and subsequent proteolysis of LEF1. Together these effects inhibit the transcriptional activation of canonical Wnt/beta-catenin target genes. Negative regulator of the Notch signaling pathway. Binds to and phosphorylates NOTCH1, thereby preventing the formation of a transcriptionally active ternary complex of NOTCH1, RBPJ/RBPSUH and MAML1. Negative regulator of the MYB family of transcription factors. Phosphorylation of MYB leads to its subsequent proteolysis while phosphorylation of MYBL1 and MYBL2 inhibits their interaction with the coactivator CREBBP. Other transcription factors may also be inhibited by direct phosphorylation of CREBBP itself. Acts downstream of IL6 and MAP3K7/TAK1 to phosphorylate STAT3, which is in turn required for activation of NLK by MAP3K7/TAK1. Upon IL1B stimulus, cooperates with ATF5 to activate the transactivation activity of C/EBP subfamily members. Phosphorylates ATF5 but also stabilizes ATF5 protein levels in a kinase-independent manner (PubMed:25512613).

    Gene: NLK

    Poids moléculaire
    58kDa
    ID gène
    51701
    UniProt
    Q9UBE8
    Pathways
    Ubiquitin Proteasome Pathway
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