PARP1 anticorps (Internal Region)

N° du produit ABIN6264040

Détail du produit anti-PARP1 anticorps

Antigène: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Épitope: Internal Region

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PARP1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificité: PARP1 Antibody detects endogenous levels of total PARP1.

Homologie: Pig,Zebrafish,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogène: A synthesized peptide derived from human PARP1, corresponding to a region within the internal amino acids.

Isotype: IgG

Information d'application

Indications d'application: WB 1:500-1:1000, IHC 1:50-1:100, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at -20 °C. Stable for 12 months from date of receipt.

Date de péremption: 12 months

Références pour anticorps anti-PARP1 (ABIN6264040)

Liu, Geng, Zhang, Wang, Zhang, Duan, Zhang: "Oligo-Porphyran Ameliorates Neurobehavioral Deficits in Parkinsonian Mice by Regulating the PI3K/Akt/Bcl-2 Pathway." dans: Marine drugs, Vol. 16, Issue 3, (2018) (PubMed).

Détails sur PARP1

Antigène: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Autre désignation: PARP1 (PARP1 Produits)

Synonymes: anticorps ADPRT, anticorps ADPRT 1, anticorps ADPRT1, anticorps ARTD1, anticorps PARP, anticorps PARP-1, anticorps PPOL, anticorps pADPRT-1, anticorps Adprt, anticorps Parp-1, anticorps 5830444G22Rik, anticorps AI893648, anticorps Adprp, anticorps Adprt1, anticorps C80510, anticorps parp-1, anticorps sPARP-1, anticorps BEST:LD21673, anticorps CG17685, anticorps CG17696, anticorps CG17718, anticorps CG40411, anticorps D.PARP, anticorps Dm.pARTa, anticorps Dmel\\CG40411, anticorps LD21673.3prime, anticorps PARP1, anticorps dPARP, anticorps parp, anticorps adprt1, anticorps padprt-1, anticorps ppol, anticorps si:dkey-206f10.3, anticorps wu:fc60f12, anticorps zgc:110092, anticorps poly(ADP-ribose) polymerase 1, anticorps poly (ADP-ribose) polymerase 1, anticorps poly (ADP-ribose) polymerase family, member 1, anticorps Poly-(ADP-ribose) polymerase, anticorps poly(ADP-ribose) polymerase 1 L homeolog, anticorps PARP1, anticorps Parp1, anticorps Parp, anticorps parp1.L, anticorps parp1

Sujet:

Description: Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks (PubMed:17177976, PubMed:18172500, PubMed:19344625, PubMed:19661379, PubMed:23230272). Mediates the poly(ADP-ribosyl)ation of APLF and CHFR (PubMed:17396150). Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. With EEF1A1 and TXK, forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production (PubMed:17177976). Required for PARP9 and DTX3L recruitment to DNA damage sites (PubMed:23230272). PARP1-dependent PARP9-DTX3L-mediated ubiquitination promotes the rapid and specific recruitment of 53BP1/TP53BP1, UIMC1/RAP80, and BRCA1 to DNA damage sites (PubMed:23230272). Mediates serine ADP-ribosylation of target proteins following interaction with HPF1, HPF1 conferring serine specificity (PubMed:28190768). Mediates the poly(ADP-ribosyl)ation of histones in a HPF1-dependent manner (PubMed:27067600). Involved in the synthesis of ATP in the nucleus, together with NMNAT1, PARG and NUDT5 (PubMed:27257257). Nuclear ATP generation is required for extensive chromatin remodeling events that are energy-consuming (PubMed:27257257).

Gene: PARP1

Poids moléculaire: 113kDa

ID gène: 142

UniProt: P09874

Pathways: Apoptose, Caspase Cascade in Apoptosis, Réparation de l'ADN, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location