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POLH anticorps (Internal Region)

POLH Reactivité: Humain, Souris, Rat WB, ELISA, IHC Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN6264337
  • Antigène Voir toutes POLH Anticorps
    POLH (Polymerase (DNA Directed), eta (POLH))
    Épitope
    • 15
    • 5
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    Internal Region
    Reactivité
    • 42
    • 11
    • 11
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain, Souris, Rat
    Hôte
    • 42
    • 3
    Lapin
    Clonalité
    • 44
    • 1
    Polyclonal
    Conjugué
    • 18
    • 4
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp POLH est non-conjugé
    Application
    • 32
    • 13
    • 13
    • 11
    • 7
    • 5
    • 4
    • 3
    • 2
    • 2
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
    Specificité
    POLH Antibody detects endogenous levels of total POLH.
    Homologie
    Sheep
    Purification
    The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
    Immunogène
    A synthesized peptide derived from human POLH, corresponding to a region within the internal amino acids.
    Isotype
    IgG
    Top Product
    Discover our top product POLH Anticorps primaire
  • Indications d'application
    WB 1:500-1:2000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    -20 °C
    Stockage commentaire
    Store at -20 °C. Stable for 12 months from date of receipt.
    Date de péremption
    12 months
  • Antigène
    POLH (Polymerase (DNA Directed), eta (POLH))
    Autre désignation
    POLH (POLH Produits)
    Synonymes
    anticorps POLH, anticorps fc50a12, anticorps wu:fc50a12, anticorps zgc:136881, anticorps zgc:152642, anticorps RAD30, anticorps RAD30A, anticorps XP-V, anticorps XPV, anticorps polymerase (DNA directed), eta S homeolog, anticorps DNA polymerase eta, anticorps polymerase (DNA directed), eta, anticorps putative DNA polymerase eta, anticorps polymerase (DNA directed), eta (RAD 30 related), anticorps polh.S, anticorps POLH, anticorps polh, anticorps Tb10.70.6190, anticorps LINJ_21_0690, anticorps Polh, anticorps LOC100443019
    Sujet

    Description: DNA polymerase specifically involved in the DNA repair by translesion synthesis (TLS) (PubMed:10385124, PubMed:11743006, PubMed:24449906). Due to low processivity on both damaged and normal DNA, cooperates with the heterotetrameric (REV3L, REV7, POLD2 and POLD3) POLZ complex for complete bypass of DNA lesions. Inserts one or 2 nucleotide(s) opposite the lesion, the primer is further extended by the tetrameric POLZ complex. In the case of 1,2-intrastrand d(GpG)-cisplatin cross-link, inserts dCTP opposite the 3' guanine (PubMed:24449906). Particularly important for the repair of UV-induced pyrimidine dimers (PubMed:10385124, PubMed:11743006). Although inserts the correct base, may cause base transitions and transversions depending upon the context. May play a role in hypermutation at immunoglobulin genes (PubMed:11376341, PubMed:14734526). Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have any lyase activity, preventing the release of the 5'-deoxyribose phosphate (5'-dRP) residue. This covalent trapping of the enzyme by the 5'-dRP residue inhibits its DNA synthetic activity during base excision repair, thereby avoiding high incidence of mutagenesis (PubMed:14630940). Targets POLI to replication foci (PubMed:12606586).

    Gene: POLH

    Poids moléculaire
    78kDa
    ID gène
    5429
    UniProt
    Q9Y253
    Pathways
    Réparation de l'ADN
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