Nucleolin anticorps
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- Antigène Voir toutes Nucleolin (NCL) Anticorps
- Nucleolin (NCL)
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Reactivité
- Humain
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp Nucleolin est non-conjugé
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)
- Fonction
- Mouse anti-Human Nucleolin Antibody Cocktail [Sodium Azide Free]
- Specificité
- Nucleoli
- Purification
- SUDHL1 cell nuclear lysate (364-5) and recombinant human protein (NCL/902) were used as the immunogen for the Nucleolin antibody cocktail.
- Immunogène
- SUDHL1 cell nuclear lysate (364-5) and recombinant human protein (NCL/902) were used as the immunogen for the Nucleolin antibody cocktail.
- Top Product
- Discover our top product NCL Anticorps primaire
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- Indications d'application
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Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 0.5-1 μg/mL
Western blot: 0.5-1 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2) - Restrictions
- For Research Use only
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- Buffer
- In 1X PBS, BSA free, sodium azide free
- Agent conservateur
- Azide free
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
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- Antigène
- Nucleolin (NCL)
- Autre désignation
- Nucleolin (NCL Produits)
- Synonymes
- anticorps C23, anticorps B530004O11Rik, anticorps D0Nds28, anticorps D1Nds28, anticorps Nucl, anticorps wu:fa12d03, anticorps zgc:152810, anticorps nucl, anticorps LOC100282923, anticorps LOC100283058, anticorps nucleolin, anticorps nucleolin S homeolog, anticorps NCL, anticorps Ncl, anticorps ncl.S, anticorps ncl, anticorps LOC100195537, anticorps LOC100282923, anticorps LOC100283058
- Sujet
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Target Description: Recognizes a protein of ~76 kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. This mAb can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.
Gene Symbol: NCL
- ID gène
- 4691
- UniProt
- P19338
- Pathways
- Ribonucleoprotein Complex Subunit Organization
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