KIT anticorps (FITC)

N° du produit ABIN638436

Détail du produit anti-KIT anticorps (FITC)

Antigène: KIT (Mast/stem Cell Growth Factor Receptor (KIT))

Reactivité: Humain, Boeuf (Vache), Non-Human Primate

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp KIT est conjugé à/à la FITC

Application: Flow Cytometry (FACS)

Specificité: The mouse monoclonal antibody 104D2 detects extracellular part of CD117 / c-Kit protooncogen.

Réactivité croisée (Details): Human, Non-Human Primates, Bovine

Purification: Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogène: MOLM-1 megakaryocytic cells

Clone: 104D2

Isotype: IgG1 kappa

Information d'application

Indications d'application: Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.

Commentaires:

The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use. No reconstitution is necessary.

Restrictions: For Research Use only

Stockage

Reconstitution: No reconstitution is necessary.

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze.
Avoid prolonged exposure to light.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Références pour anticorps anti-KIT (FITC) (ABIN638436)

Stevenson, McGlynn, Hodge, McLinden, George, Davies, Shiels: "Isolation, characterization, and differentiation of thy1.1-sorted pancreatic adult progenitor cell populations." dans: Stem cells and development, Vol. 18, Issue 10, pp. 1389-98, (2009) (PubMed).

Nagano, Yamashita, Hamada, Ohneda, Kimura, Nakagawa, Shibuya, Yoshikawa, Ohneda: "Identification of functional endothelial progenitor cells suitable for the treatment of ischemic tissue using human umbilical cord blood." dans: Blood, Vol. 110, Issue 1, pp. 151-60, (2007) (PubMed).

Wihlidal, Varga, Pfeilstöcker, Karlic: "Expression and functional significance of osteocalcin splicing in disease progression of hematological malignancies." dans: Leukemia research, Vol. 30, Issue 10, pp. 1241-8, (2006) (PubMed).

Blair, Sutherland: "Primitive acute myeloid leukemia cells with long-term proliferative ability in vitro and in vivo lack surface expression of c-kit (CD117)." dans: Experimental hematology, Vol. 28, Issue 6, pp. 660-71, (2000) (PubMed).

Yoshino, Ami, Terao, Tashiro, Honda: "Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies." dans: Experimental animals / Japanese Association for Laboratory Animal Science, Vol. 49, Issue 2, pp. 97-110, (2000) (PubMed).

Broudy, Lin, Liles, Corey, OLaughlin, Mou, Linnekin: "Signaling via Src family kinases is required for normal internalization of the receptor c-Kit." dans: Blood, Vol. 94, Issue 6, pp. 1979-86, (1999) (PubMed).

Broudy, Lin, Bühring, Komatsu, Kavanagh: "Analysis of c-kit receptor dimerization by fluorescence resonance energy transfer." dans: Blood, Vol. 91, Issue 3, pp. 898-906, (1998) (PubMed).

Rappold, Ziegler, Köhler, Marchetto, Rosnet, Birnbaum, Simmons, Zannettino, Hill, Neu, Knapp, Alitalo, Alitalo, Ullrich, Kanz, Bühring: "Functional and phenotypic characterization of cord blood and bone marrow subsets expressing FLT3 (CD135) receptor tyrosine kinase." dans: Blood, Vol. 90, Issue 1, pp. 111-25, (1997) (PubMed).

Détails sur KIT

Antigène: KIT (Mast/stem Cell Growth Factor Receptor (KIT))

Autre désignation: CD117 (KIT Produits)

Synonymes: anticorps C-Kit, anticorps CD117, anticorps PBT, anticorps SCFR, anticorps Bs, anticorps Fdc, anticorps Gsfsco1, anticorps Gsfsco5, anticorps Gsfsow3, anticorps SCO1, anticorps SCO5, anticorps SOW3, anticorps Ssm, anticorps Tr-kit, anticorps W, anticorps c-KIT, anticorps c-kit, anticorps MGF, anticorps KIT, anticorps C-KIT, anticorps kit, anticorps receptor, anticorps sparse, anticorps Xkl-1, anticorps kit-a, anticorps kit-b, anticorps kl1-A, anticorps krk1, anticorps pbt, anticorps scfr, anticorps KIT proto-oncogene receptor tyrosine kinase, anticorps v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog a, anticorps KIT proto-oncogene receptor tyrosine kinase S homeolog, anticorps KIT, anticorps Kit, anticorps kita, anticorps kit, anticorps kit.S

Sujet: KIT proto-oncogene, receptor tyrosine kinase,CD117 / c-Kit (stem cell factor receptor) is a 145 kDa receptor tyrosine kinase that regulates cell proliferation, adhesion, chemotaxis, apoptosis and other cell processes. Mutations of CD117 / c-Kit can lead to growth and progression of tumours. After binding of its ligand, SCF (stem cell factor), CD117 / c-Kit is autophosphorylated on its intracellular domains and activated. CD117 is expressed on pluripotent hematopoietic progenitor cells, mast cells and various cancer cells, e.g. acute myeloid leukemia cells.,c-Kit, PBT, SCFR

ID gène: 3815

UniProt: P10721

Pathways: Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Sensory Perception of Sound, Stem Cell Maintenance, Production of Molecular Mediator of Immune Response, Regulation of long-term Neuronal Synaptic Plasticity