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Sonic Hedgehog anticorps (C-Term)

Name: Sonic Hedgehog anticorps (C-Term)
Brand: antibodies-online
SKU: ABIN6391361
Price: 779.48 EUR
Availability: InStock

Verified SHH Reactivité: Humain, Souris WB, ELISA, IF, FACS Hôte: Chèvre Polyclonal unconjugated

ABIN6391361 (2µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer). (Sonic Hedgehog anticorps (C-Term))

ABIN6391361 Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0. (Sonic Hedgehog anticorps (C-Term))

ABIN6391361 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0. (Sonic Hedgehog anticorps (C-Term))

ABIN6391361 Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0. (Sonic Hedgehog anticorps (C-Term))

ABIN6391361 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0. (Sonic Hedgehog anticorps (C-Term))

FACS

5 images

N° du produit ABIN6391361

Fiche de données

Antigène Voir toutes Sonic Hedgehog (SHH) Anticorps

Sonic Hedgehog (SHH)
Épitope
15

12

9

5

4

4

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1
C-Term
Reactivité
65

49

37

17

10

6

6

5

2

2

2

1

1

1

1

1

1

1
Humain, Souris
Hôte
73

12

8

1
Chèvre
Anticorps secondaires appropriés
Clonalité
67

27
Polyclonal
Conjugué
51

8

4

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Cet anticorp Sonic Hedgehog est non-conjugé
Application
68

27

23

14

13

11

8

6

6

6

5

4

3

1
Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Fonction

SHH

Séquence

DSEALHPLGM AVK

Réactivité croisée

Chien, Humain, Rat

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Classe de qualité

Verified

Isotype

IgG

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SHH Reactivité: Humain, Rat WB, IHC (p) Hôte: Lapin Polyclonal unconjugated

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SHH Reactivité: Souris WB, IHC, IP, ICC Hôte: Lapin Polyclonal unconjugated

Indications d'application

Western Blot: Approx 48 kDa band observed in Human Liver lysates (calculated MW of 49.6 kDa according to NP_000184.1 Recommended concentration: 1-3 μg/mL. Primary incubation 1 hour at room temperature. Preliminary testing was unsuccessful on Rat Liver for t
Peptide ELISA: antibody detection limit dilution 1:32000.

Commentaires

Immunofluorescence: Strong expression of the protein seen in the membranes of HeLa, U251 and NIH3T3 cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concen

Restrictions

For Research Use only
Format

Liquid

Concentration

0.5 mg/mL

Buffer

Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur

Sodium azide

Précaution d'utilisation

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation

Minimize freezing and thawing.

Stock

-20 °C

Stockage commentaire

Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
Antigène

Sonic Hedgehog (SHH)

Autre désignation

SHH (SHH Produits)

Synonymes

anticorps HHG1, anticorps HLP3, anticorps HPE3, anticorps MCOPCB5, anticorps SMMCI, anticorps TPT, anticorps TPTPS, anticorps 9530036O11Rik, anticorps Dsh, anticorps Hhg1, anticorps Hx, anticorps Hxl3, anticorps M100081, anticorps fc83d08, anticorps shh, anticorps syu, anticorps vhh-1, anticorps vhh1, anticorps wu:fc83d08, anticorps Xhh, anticorps hedgehog, anticorps xshh, anticorps SHH, anticorps twh, anticorps twhh, anticorps sonic hedgehog, anticorps sonic hedgehog a, anticorps sonic hedgehog L homeolog, anticorps sonic hedgehog protein A, anticorps sonic hedgehog b, anticorps SHH, anticorps Shh, anticorps shha, anticorps shh.L, anticorps shh, anticorps shhb

Sujet

SHH, sonic hedgehog homolog (Drosophila), HGNC:10848, HHG1, HLP3, HPE3, SMMCI , sonic hedgehog

ID gène

6469

NCBI Accession

NP_000184

Pathways

Signalisation Hedgehog, Dopaminergic Neurogenesis, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development