Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Purification
This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogène
This OPN-a/b antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 14-40 amino acids from the N-terminal region of human OPN-a/b.
Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
4 °C,-20 °C
Stockage commentaire
Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Date de péremption
6 months
Tu, Li, Zeng, Deng, Gao, Xiao, Luo, Jiang, Li, Lei: "MicroRNA-127-5p regulates osteopontin expression and osteopontin-mediated proliferation of human chondrocytes." dans: Scientific reports, Vol. 6, pp. 25032, (2018) (PubMed).
Wang, Wang, Zhang, Deng, Long: "NEAT1/miR-181c Regulates Osteopontin (OPN)-Mediated Synoviocyte Proliferation in Osteoarthritis." dans: Journal of cellular biochemistry, Vol. 118, Issue 11, pp. 3775-3784, (2018) (PubMed).
Liang, Xu, Zhou, Liu, Ge, Chen, Tu: "MALAT1/miR-127-5p Regulates Osteopontin (OPN)-Mediated Proliferation of Human Chondrocytes Through PI3K/Akt Pathway." dans: Journal of cellular biochemistry, Vol. 119, Issue 1, pp. 431-439, (2018) (PubMed).
Li, Jiang, Wang, Deng, Zeng, Tu, Li, Xiao, Gao, Luo, Lei: "Osteopontin Promotes Expression of Matrix Metalloproteinase 13 through NF-κB Signaling in Osteoarthritis." dans: BioMed research international, Vol. 2016, pp. 6345656, (2016) (PubMed).
Antigène
OPN-A,b
Sujet
Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity (By similarity).