ALK anticorps (AA 200-335)

N° du produit ABIN6654202

Détail du produit anti-ALK anticorps

Antigène: ALK (Anaplastic Lymphoma Receptor tyrosine Kinase (ALK))

Épitope: AA 200-335

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ALK est non-conjugé

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: Purified

Pureté: Protein G affinity

Immunogène: A portion of amino acids 200-335 from the human protein was used as the immunogen for this ALK antibody.

Clone: ALK-1504

Isotype: IgG2b kappa

Information d'application

Indications d'application: The stated application concentrations are suggested starting amounts. Titration of the ALK antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. IHC (FFPE): 1-2 μg/mL for 30 min at RT

Restrictions: For Research Use only

Stockage

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store the recombinant ALK antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détails sur ALK

Antigène: ALK (Anaplastic Lymphoma Receptor tyrosine Kinase (ALK))

Autre désignation: ALK / Anaplastic Lymphoma Kinase (ALK Produits)

Synonymes: anticorps Tcrz, anticorps CD246, anticorps NBLST3, anticorps ALK, anticorps anaplastic lymphoma kinase, anticorps anaplastic lymphoma receptor tyrosine kinase, anticorps ALK receptor tyrosine kinase, anticorps Alk, anticorps ALK

Sujet: The wild-type anaplastic lymphoma kinase (ALK) protein is a 200 kDa transmembrane receptor tyrosine kinase. Its expression is restricted to a few scattered cells in the nervous system (some glial cells and neurons, and a few endothelial cells and pericytes. The hybrid gene,  NPM-ALK, created by the t(2,5)(p23,q35) chromosomal translocation encodes part of the nucleolar phosphoprotein, nucleophosmin (NPM), joined to the entire cytoplasmic portion of the anaplastic lymphoma kinase (ALK) receptor tyrosine kinase. As a consequence, the ALK gene comes under the control of the NPM promoter, which induces a permanent and ubiquitous transcription of the NPM-ALK hybrid gene, resulting in the production of a 80 kDa NPM-ALK chimeric protein. This translocation is found in anaplastic large cell lymphomas (ALCL). Reportedly, expression of ALK indicates a better prognosis. Approximately 5 % -10 % of non-small cell lung carcinomas also express ALK protein producing a cytoplasmic staining pattern. This MAb also reacts with blood vessels that serves as an internal positive control.

Pathways: Signalisation RTK