TLR3 anticorps

N° du produit ABIN6655315

N° du produit (Fournisseur): 200-301-i24

Détail du produit anti-TLR3 anticorps

Antigène: TLR3 (Toll-Like Receptor 3 (TLR3))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp TLR3 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunofluorescence (IF)

N° du produit (Fournisseur): 200-301-i24

Fournisseur: Rockland

Fonction: TLR3 Antibody

Réactivité croisée (Details): A BLAST analysis was used to suggest cross-reactivity with Anti-TLR3 from dog, human and mouse based on 100 % homology with the immunizing sequence.

Purification: Anti-TLR3 Antibody was purified by Protein G chromatography.

Immunogène: TLR3 Antibody was produced in mice prepared by repeated immunizations with a peptide corresponding to internal amino acid sequences in the human protein TLR3.

Clone: 40C1285-6

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Immunoprecipitation_Dilution: 2 μg/10^6 cells

Immunohistochemistry_Dilution: 10 μg/mL

Flow_Cytometry_Dilution: 2-4 μg/10^6 cells

Western_Blot_Dilution: 1-3 μg/mL

Commentaires:

Anti-TLR3 Antibody is tested for use in WB, Flow, Flow-IC, ICC/IF, IHC, IHC-P, IP, and Bioactivity. Expect a band approximately 120kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.05 % BSA

Preservative: 0.05 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur TLR3

Antigène: TLR3 (Toll-Like Receptor 3 (TLR3))

Autre désignation: TLR3 (TLR3 Produits)

Sujet:

Synonyms: Toll-like receptor 3, CD283

Background: Anti-TLR3 Antibody detects human TLR3. The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules and could constitute an important and unrecognized component of innate immunity in humans. The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. TLRs characterized so far activate the MyD88/interleukin-1 receptor-associated kinase (IRAK) signaling pathway. Ten human homologs of TLRs (TLR1-10) have been described. TLR3 cDNA codes for a protein with approximate molecular weight of 120 kDa. TLR3 has a restricted expression pattern being expressed in dendritic cells (DC). TLR3 mRNA expression was detected by in situ hybridization in DC and lymph nodes . The expression of TLR3 in a single cell type may indicate a specific role for this molecule in a restricted setting. Anti-TLR3 Antibody is ideal for investigators involved in cytokines and growth factor research.

Gene Name: TLR3

ID gène: 7098

NCBI Accession: NP_003256

UniProt: O15455

Pathways: Signalisation TLR, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades