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POLR2A/RPB1 anticorps (pSer2)

POLR2A Reactivité: Humain WB, ELISA, ChIP, FM, CUT&RUN Hôte: Souris Monoclonal unconjugated
N° du produit ABIN6655366
  • Antigène Voir toutes POLR2A/RPB1 (POLR2A) Anticorps
    POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))
    Épitope
    • 17
    • 15
    • 11
    • 7
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pSer2
    Reactivité
    • 88
    • 47
    • 39
    • 6
    • 6
    • 6
    • 5
    • 5
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    Humain
    Hôte
    • 72
    • 16
    • 1
    • 1
    • 1
    Souris
    Clonalité
    • 56
    • 35
    Monoclonal
    Conjugué
    • 53
    • 6
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 1
    • 1
    Cet anticorp POLR2A/RPB1 est non-conjugé
    Application
    • 48
    • 26
    • 23
    • 21
    • 12
    • 10
    • 7
    • 7
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Chromatin Immunoprecipitation (ChIP), Fluorescence Microscopy (FM), Cleavage Under Targets and Release Using Nuclease (CUT&RUN)
    Purification
    Anti-Pol II pS2 Antibody was purified by Protein A chromatography. This antibody is specific for Pol II protein phosphorylated Ser2. Cross-reactivity with Pol II pS2 from other sources has not been determined.
    Immunogène

    Immunogen: Anti-Pol II S2p Antibody was produced in mice by repeated immunization with the YSPTSPS repeat in the B1 subunit of RNA polymerase II phosphorylated at Ser2 of the repeated sequence.

    Immunogen Type: Peptide

    Isotype
    IgG1
    Top Product
    Discover our top product POLR2A Anticorps primaire
  • Indications d'application
    The rabbit anti-PolII (pSer2) antibody ABIN6655366 is suitable for use in ChIP, CUT&RUN, ELISA, immunofluorescence microscopy, and western blot. Specific conditions for each assay should be optimized by the end user. Expect a band of approximately 225 kDa in western blot in the appropriate cell lysate or extract. General ABIN6655366 dilution recommendations for different applications are as follows:
    • ChIP: 1 µg/ChIP
    • ELISA: 1:3,000
    • IF: 1:500
    • WB: 1:1,000
    • CUT&RUN: 1:100
    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer

    Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

    0.02 % (w/v) Sodium Azide

    Stabilizer: None

    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    RT,4 °C,-20 °C
    Stockage commentaire
    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
  • Antigène
    POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))
    Autre désignation
    Pol II (POLR2A Produits)
    Synonymes
    anticorps NCU02103.1, anticorps 19.m02399, anticorps POLR2A, anticorps 220kDa, anticorps Rpb1, anticorps Rpo2-1, anticorps POLR2, anticorps POLRA, anticorps RPB1, anticorps RPBh1, anticorps RPO2, anticorps RPOL2, anticorps RpIILS, anticorps hRPB220, anticorps hsRPB1, anticorps DNA-directed RNA polymerase II largest subunit, anticorps DNA-directed RNA polymerase II subunit RPB1, anticorps RNA polymerase II large subunit Rpb1, anticorps RNA polymerase II subunit A, anticorps DNA-directed RNA polymerase II largest subunit, putative, anticorps polymerase (RNA) II (DNA directed) polypeptide A, 220kDa, anticorps polymerase (RNA) II (DNA directed) polypeptide A, anticorps NCU02103, anticorps BBOV_I003360, anticorps EDI_169920, anticorps CMU_000800, anticorps SJAG_01713, anticorps MGYG_01527, anticorps POLR2A, anticorps TP03_0185, anticorps EBI_22514, anticorps CGB_E5020W, anticorps polr2a, anticorps Polr2a
    Sujet

    Synonyms: DNA-directed RNA polymerase II subunit RPB1, RNA polymerase II subunit B1, DNA directed RNA polymerase II subunit A, DNA-directed RNA polymerase III largest subunit, RNA-directed RNA polymerase II subunit RPB1

    Background: RNA polymerase II (pol II) is a key enzyme in the regulation and control of gene transcription. It is able to unwind the DNA double helix, synthesize RNA, and proofread the result. Pol II is a complex enzyme, consisting of 12 subunits, of which the B1 subunit (UniProt/Swiss-Prot entry P24928) is the largest. Together with the second largest subunit, B1 forms the catalytic core of the RNA polymerase II transcription machinery. Anti-Pol II S2p Antibody is ideal for research in Gene Expression, Transcription, and Genetics.

    Gene Name: POLR2A

    ID gène
    5430
    NCBI Accession
    NP_000928
    UniProt
    P24928
    Pathways
    Regulatory RNA Pathways
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