POLR2A/RPB1 anticorps (pSer2)
-
- Antigène Voir toutes POLR2A/RPB1 (POLR2A) Anticorps
- POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))
-
Épitope
- pSer2
-
Reactivité
- Humain
-
Hôte
- Souris
-
Clonalité
- Monoclonal
-
Conjugué
- Cet anticorp POLR2A/RPB1 est non-conjugé
-
Application
- Western Blotting (WB), ELISA, Chromatin Immunoprecipitation (ChIP), Fluorescence Microscopy (FM), Cleavage Under Targets and Release Using Nuclease (CUT&RUN)
- Purification
- Anti-Pol II pS2 Antibody was purified by Protein A chromatography. This antibody is specific for Pol II protein phosphorylated Ser2. Cross-reactivity with Pol II pS2 from other sources has not been determined.
- Immunogène
-
Immunogen: Anti-Pol II S2p Antibody was produced in mice by repeated immunization with the YSPTSPS repeat in the B1 subunit of RNA polymerase II phosphorylated at Ser2 of the repeated sequence.
Immunogen Type: Peptide
- Isotype
- IgG1
- Top Product
- Discover our top product POLR2A Anticorps primaire
-
-
- Indications d'application
-
The rabbit anti-PolII (pSer2) antibody ABIN6655366 is suitable for use in ChIP, CUT&RUN, ELISA, immunofluorescence microscopy, and western blot. Specific conditions for each assay should be optimized by the end user. Expect a band of approximately 225 kDa in western blot in the appropriate cell lysate or extract.
General ABIN6655366 dilution recommendations for different applications are as follows:
- ChIP: 1 µg/ChIP
- ELISA: 1:3,000
- IF: 1:500
- WB: 1:1,000
- CUT&RUN: 1:100
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Buffer
-
Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
0.02 % (w/v) Sodium AzideStabilizer: None
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- RT,4 °C,-20 °C
- Stockage commentaire
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
-
- Antigène
- POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))
- Autre désignation
- Pol II (POLR2A Produits)
- Synonymes
- anticorps NCU02103.1, anticorps 19.m02399, anticorps POLR2A, anticorps 220kDa, anticorps Rpb1, anticorps Rpo2-1, anticorps POLR2, anticorps POLRA, anticorps RPB1, anticorps RPBh1, anticorps RPO2, anticorps RPOL2, anticorps RpIILS, anticorps hRPB220, anticorps hsRPB1, anticorps DNA-directed RNA polymerase II largest subunit, anticorps DNA-directed RNA polymerase II subunit RPB1, anticorps RNA polymerase II large subunit Rpb1, anticorps RNA polymerase II subunit A, anticorps DNA-directed RNA polymerase II largest subunit, putative, anticorps polymerase (RNA) II (DNA directed) polypeptide A, 220kDa, anticorps polymerase (RNA) II (DNA directed) polypeptide A, anticorps NCU02103, anticorps BBOV_I003360, anticorps EDI_169920, anticorps CMU_000800, anticorps SJAG_01713, anticorps MGYG_01527, anticorps POLR2A, anticorps TP03_0185, anticorps EBI_22514, anticorps CGB_E5020W, anticorps polr2a, anticorps Polr2a
- Sujet
-
Synonyms: DNA-directed RNA polymerase II subunit RPB1, RNA polymerase II subunit B1, DNA directed RNA polymerase II subunit A, DNA-directed RNA polymerase III largest subunit, RNA-directed RNA polymerase II subunit RPB1
Background: RNA polymerase II (pol II) is a key enzyme in the regulation and control of gene transcription. It is able to unwind the DNA double helix, synthesize RNA, and proofread the result. Pol II is a complex enzyme, consisting of 12 subunits, of which the B1 subunit (UniProt/Swiss-Prot entry P24928) is the largest. Together with the second largest subunit, B1 forms the catalytic core of the RNA polymerase II transcription machinery. Anti-Pol II S2p Antibody is ideal for research in Gene Expression, Transcription, and Genetics.
Gene Name: POLR2A
- ID gène
- 5430
- NCBI Accession
- NP_000928
- UniProt
- P24928
- Pathways
- Regulatory RNA Pathways
-