POLR2A/RPB1 anticorps (pSer5)

N° du produit ABIN6655367

Détail du produit anti-POLR2A/RPB1 anticorps

Antigène: POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))

Épitope: pSer5

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp POLR2A/RPB1 est non-conjugé

Application: Western Blotting (WB), ELISA, Chromatin Immunoprecipitation (ChIP), Fluorescence Microscopy (FM), Cleavage Under Targets and Release Using Nuclease (CUT&RUN)

Purification: Anti-Pol II pS5 Antibody was purified by Protein A chromatography. This antibody is specific for Pol II protein phosphorylated Ser5. Cross-reactivity with Pol II pS5 from other sources has not been determined.

Immunogène:

Immunogen: Anti-Pol II S5p Antibody was produced in mice by repeated immunization with the YSPTSPS repeat in the B1 subunit of RNA polymerase II phosphorylated at Ser5 of the repeated sequence.

Immunogen Type: Peptide

Isotype: IgG1

Information d'application

Indications d'application: The rabbit anti-PolII (pSer5) antibody ABIN6655367 is suitable for use in ChIP, CUT&RUN, ELISA, immunofluorescence microscopy, and western blot. Specific conditions for each assay should be optimized by the end user. Expect a band of approximately 225 kDa in western blot in the appropriate cell lysate or extract. General ABIN6655367 dilution recommendations for different applications are as follows:

• ChIP: 1 µg/ChIP
• ELISA: 1:3,000
• IF: 1:500
• WB: 1:1,000
• CUT&RUN: 1:100

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

0.02 % (w/v) Sodium Azide

Stabilizer: None

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Détails sur POLR2A/RPB1

Antigène: POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))

Autre désignation: Pol II (POLR2A Produits)

Synonymes: anticorps NCU02103.1, anticorps 19.m02399, anticorps POLR2A, anticorps 220kDa, anticorps Rpb1, anticorps Rpo2-1, anticorps POLR2, anticorps POLRA, anticorps RPB1, anticorps RPBh1, anticorps RPO2, anticorps RPOL2, anticorps RpIILS, anticorps hRPB220, anticorps hsRPB1, anticorps DNA-directed RNA polymerase II largest subunit, anticorps DNA-directed RNA polymerase II subunit RPB1, anticorps RNA polymerase II large subunit Rpb1, anticorps RNA polymerase II subunit A, anticorps DNA-directed RNA polymerase II largest subunit, putative, anticorps polymerase (RNA) II (DNA directed) polypeptide A, 220kDa, anticorps polymerase (RNA) II (DNA directed) polypeptide A, anticorps NCU02103, anticorps BBOV_I003360, anticorps EDI_169920, anticorps CMU_000800, anticorps SJAG_01713, anticorps MGYG_01527, anticorps POLR2A, anticorps TP03_0185, anticorps EBI_22514, anticorps CGB_E5020W, anticorps polr2a, anticorps Polr2a

Sujet:

Synonyms: DNA-directed RNA polymerase II subunit RPB1, RNA polymerase II subunit B1, DNA directed RNA polymerase II subunit A, DNA-directed RNA polymerase III largest subunit, RNA-directed RNA polymerase II subunit RPB1

Background: RNA polymerase II (pol II) is a key enzyme in the regulation and control of gene transcription. It is able to unwind the DNA double helix, synthesize RNA, and proofread the result. Pol II is a complex enzyme, consisting of 12 subunits, of which the B1 subunit is the largest. Together with the second largest subunit, B1 forms the catalytic core of the RNA polymerase II transcription machinery. Anti-Pol II S5p Antibody is ideal for research in Gene Expression, Transcription and Genetics.

Gene Name: POLR2A

ID gène: 5430

NCBI Accession: NP_000928

UniProt: P24928

Pathways: Regulatory RNA Pathways